The Arabidopsis thaliana basic helix-loop-helix Leu zipper transcription factor MYC2/JIN1 differentially regulates jasmonate (JA)-responsive pathogen defense and wound response genes. This study reveals new roles for MYC2 in modulating diverse JA functions. MYC2 negatively regulates tryptophan (Trp) and Trp-derived secondary metabolism, including indole glucosinolate biosynthesis, during JA signaling. It also positively regulates JA-mediated resistance to insect pests and tolerance to oxidative stress, possibly via enhanced ascorbate redox cycling and flavonoid biosynthesis. Analysis of MYC2 cis-binding elements and expression of MYC2-regulated genes in T-DNA insertion lines of a subset of MYC2-regulated transcription factors (TFs) suggests that MYC2 modulates JA responses via differential regulation of an intermediate spectrum of TFs with activating or repressing roles in JA signaling. MYC2 also negatively regulates its own expression, which may be one of the mechanisms used in fine-tuning JA signaling. These results provide new insights into the function of MYC2 and the transcriptional coordination of the JA signaling pathway. MYC2 modulates gene expression in a JA-dependent manner, negatively regulating Trp metabolism during JA signaling and positively regulating JA-dependent IG biosynthesis. MYC2 is a positive regulator of JA-mediated flavonoid biosynthesis and is required for the sensitivity of root elongation to auxin transport inhibitors. MYC2 positively regulates oxidative stress tolerance in a JA-dependent manner and resistance to insect herbivory. MYC2 preferentially binds to an extended G-box motif and negatively regulates its own transcription. MYC2 modulates the JA-dependent transcription of TF genes, suggesting a hierarchical model in which MYC2 positively or negatively modulates the JA-dependent transcription of other TF genes, which in turn may control the JA-dependent transcription of downstream JA response genes. These findings highlight the complex regulatory network involving MYC2 in JA signaling and its role in modulating diverse JA functions in Arabidopsis.The Arabidopsis thaliana basic helix-loop-helix Leu zipper transcription factor MYC2/JIN1 differentially regulates jasmonate (JA)-responsive pathogen defense and wound response genes. This study reveals new roles for MYC2 in modulating diverse JA functions. MYC2 negatively regulates tryptophan (Trp) and Trp-derived secondary metabolism, including indole glucosinolate biosynthesis, during JA signaling. It also positively regulates JA-mediated resistance to insect pests and tolerance to oxidative stress, possibly via enhanced ascorbate redox cycling and flavonoid biosynthesis. Analysis of MYC2 cis-binding elements and expression of MYC2-regulated genes in T-DNA insertion lines of a subset of MYC2-regulated transcription factors (TFs) suggests that MYC2 modulates JA responses via differential regulation of an intermediate spectrum of TFs with activating or repressing roles in JA signaling. MYC2 also negatively regulates its own expression, which may be one of the mechanisms used in fine-tuning JA signaling. These results provide new insights into the function of MYC2 and the transcriptional coordination of the JA signaling pathway. MYC2 modulates gene expression in a JA-dependent manner, negatively regulating Trp metabolism during JA signaling and positively regulating JA-dependent IG biosynthesis. MYC2 is a positive regulator of JA-mediated flavonoid biosynthesis and is required for the sensitivity of root elongation to auxin transport inhibitors. MYC2 positively regulates oxidative stress tolerance in a JA-dependent manner and resistance to insect herbivory. MYC2 preferentially binds to an extended G-box motif and negatively regulates its own transcription. MYC2 modulates the JA-dependent transcription of TF genes, suggesting a hierarchical model in which MYC2 positively or negatively modulates the JA-dependent transcription of other TF genes, which in turn may control the JA-dependent transcription of downstream JA response genes. These findings highlight the complex regulatory network involving MYC2 in JA signaling and its role in modulating diverse JA functions in Arabidopsis.