This section of the article provides supplemental information on the mechanisms by which rapamycin extends lifespan in the fruit fly *Drosophila melanogaster*. Key findings include: 1. **Feeding Behavior**: Rapamycin does not significantly affect the feeding behavior of flies, as indicated by the proportion of feeding events and the amount of food consumed. 2. **Lifespan Extension**: Rapamycin extends the median and maximum lifespans of different laboratory strains and a sterile mutant, with no significant effect on Wolbachia status. The extension is also observed in long-lived sterile females carrying the ovoD mutation. 3. **Fecundity**: Rapamycin reduces egg-laying in various genetic backgrounds, but this reduction is independent of Wolbachia status. 4. **Survival After Stress**: Rapamycin improves survival after paraquat injection, suggesting a protective effect against oxidative stress. 5. **Atg5 Knockdown**: Knockdown of Atg5 using RNAi significantly reduces Atg5 transcript levels, indicating the efficacy of the knockdown. 6. **S6K Phosphorylation**: Rapamycin treatment reduces S6K phosphorylation in insulin-like signaling (IIS) mutants, suggesting a potential mechanism for lifespan extension. 7. **Dietary Restriction**: Rapamycin extends lifespan in flies on different food concentrations, indicating a general effect across various dietary conditions. The supplemental information also includes detailed experimental procedures, statistical analyses, and references to support the findings.This section of the article provides supplemental information on the mechanisms by which rapamycin extends lifespan in the fruit fly *Drosophila melanogaster*. Key findings include: 1. **Feeding Behavior**: Rapamycin does not significantly affect the feeding behavior of flies, as indicated by the proportion of feeding events and the amount of food consumed. 2. **Lifespan Extension**: Rapamycin extends the median and maximum lifespans of different laboratory strains and a sterile mutant, with no significant effect on Wolbachia status. The extension is also observed in long-lived sterile females carrying the ovoD mutation. 3. **Fecundity**: Rapamycin reduces egg-laying in various genetic backgrounds, but this reduction is independent of Wolbachia status. 4. **Survival After Stress**: Rapamycin improves survival after paraquat injection, suggesting a protective effect against oxidative stress. 5. **Atg5 Knockdown**: Knockdown of Atg5 using RNAi significantly reduces Atg5 transcript levels, indicating the efficacy of the knockdown. 6. **S6K Phosphorylation**: Rapamycin treatment reduces S6K phosphorylation in insulin-like signaling (IIS) mutants, suggesting a potential mechanism for lifespan extension. 7. **Dietary Restriction**: Rapamycin extends lifespan in flies on different food concentrations, indicating a general effect across various dietary conditions. The supplemental information also includes detailed experimental procedures, statistical analyses, and references to support the findings.