supplementary materials for "mechanosensing regulates tissue repair program in macrophages" include figures s1 to s5, table s1, and movies s1 to s4. figure s1 shows mechanical properties of collagen gels and pa hydrogels, including stiffness measurements, scanning electron microscopy images, pore sizes, and gene expression data. figure s2 demonstrates that fibroblast mechanosensing is integrin- and non-muscle myosin ii-dependent, with gene expression data under different conditions. figure s3 shows minimal expression of collagen-binding integrins in macrophages, based on rna-seq analysis. figure s4 indicates that yap signaling and mechanosensitive ion channels are not required for macrophage mechanosensing, with gene expression data under various treatments. figure s5 shows that a mechanosensitive gene expression program is induced during tissue repair in vivo, with flow cytometry analysis of fizz1 and arg1 expression. table s1 lists primers used for qpcr analysis. movies s1 to s4 show macrophages in 3d collagen gels under different conditions. the data are presented as mean ± sd, with statistical analyses including student's t test and two-way anova. p-values indicate significance levels.supplementary materials for "mechanosensing regulates tissue repair program in macrophages" include figures s1 to s5, table s1, and movies s1 to s4. figure s1 shows mechanical properties of collagen gels and pa hydrogels, including stiffness measurements, scanning electron microscopy images, pore sizes, and gene expression data. figure s2 demonstrates that fibroblast mechanosensing is integrin- and non-muscle myosin ii-dependent, with gene expression data under different conditions. figure s3 shows minimal expression of collagen-binding integrins in macrophages, based on rna-seq analysis. figure s4 indicates that yap signaling and mechanosensitive ion channels are not required for macrophage mechanosensing, with gene expression data under various treatments. figure s5 shows that a mechanosensitive gene expression program is induced during tissue repair in vivo, with flow cytometry analysis of fizz1 and arg1 expression. table s1 lists primers used for qpcr analysis. movies s1 to s4 show macrophages in 3d collagen gels under different conditions. the data are presented as mean ± sd, with statistical analyses including student's t test and two-way anova. p-values indicate significance levels.