The supplementary materials for the article "Mechanosensing regulates tissue repair program in macrophages" by Matthew L. Meizlish et al. include detailed figures, tables, and movies that support the main findings of the study. The figures (S1 to S5) provide insights into the mechanical properties of collagen and PA hydrogels, the role of integrins and non-muscle myosin II in fibroblast mechanosensing, the minimal expression of collagen-binding integrins in macrophages, and the independence of YAP signaling and mechanosensitive ion channels in macrophage mechanosensing. Table S1 lists the primers used for qPCR analysis. The movies (S1 to S4) visually demonstrate the behavior of macrophages in 3D low- and high-collagen gels, as well as the effects of CSF1 on macrophage behavior. These supplementary materials enhance the understanding of how mechanical cues influence macrophage function and tissue repair.The supplementary materials for the article "Mechanosensing regulates tissue repair program in macrophages" by Matthew L. Meizlish et al. include detailed figures, tables, and movies that support the main findings of the study. The figures (S1 to S5) provide insights into the mechanical properties of collagen and PA hydrogels, the role of integrins and non-muscle myosin II in fibroblast mechanosensing, the minimal expression of collagen-binding integrins in macrophages, and the independence of YAP signaling and mechanosensitive ion channels in macrophage mechanosensing. Table S1 lists the primers used for qPCR analysis. The movies (S1 to S4) visually demonstrate the behavior of macrophages in 3D low- and high-collagen gels, as well as the effects of CSF1 on macrophage behavior. These supplementary materials enhance the understanding of how mechanical cues influence macrophage function and tissue repair.