MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and have emerged as promising biomarkers for pancreatic cancer (PC). This review discusses the role of miRNAs in the diagnosis, prognosis, and therapeutic targeting of PC. miRNAs, including exosomal miRNAs, are being explored for their potential as diagnostic and prognostic indicators due to their stability and ease of detection in biofluids. Recent studies have identified several miRNAs, such as miR-21, miR-155, and miR-221, as potential biomarkers for PC. These miRNAs are dysregulated in PC tissues and biofluids and show promise in distinguishing cancer patients from healthy individuals. Additionally, miRNAs have therapeutic implications, with some being targeted for cancer treatment. However, challenges remain in standardizing miRNA analysis and identifying reliable biomarkers. The review highlights the need for further research to address these challenges and improve the accuracy of miRNA-based diagnostics and therapies for PC.MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and have emerged as promising biomarkers for pancreatic cancer (PC). This review discusses the role of miRNAs in the diagnosis, prognosis, and therapeutic targeting of PC. miRNAs, including exosomal miRNAs, are being explored for their potential as diagnostic and prognostic indicators due to their stability and ease of detection in biofluids. Recent studies have identified several miRNAs, such as miR-21, miR-155, and miR-221, as potential biomarkers for PC. These miRNAs are dysregulated in PC tissues and biofluids and show promise in distinguishing cancer patients from healthy individuals. Additionally, miRNAs have therapeutic implications, with some being targeted for cancer treatment. However, challenges remain in standardizing miRNA analysis and identifying reliable biomarkers. The review highlights the need for further research to address these challenges and improve the accuracy of miRNA-based diagnostics and therapies for PC.