This study investigates the role of microRNAs (miRNAs) in stress responses in Arabidopsis thaliana under high-salinity, drought, and cold conditions. Using microarray data, 14 stress-inducible miRNAs were identified, including 10 high-salinity-, 4 drought-, and 10 cold-regulated miRNAs. miR168, miR171, and miR396 responded to all three stresses. RT-PCR analysis showed cross-talk among the signaling pathways of these stresses. Stress-related elements in miRNA promoter regions supported these findings. The study suggests that miRNAs are important regulators in stress responses, with some miRNAs involved in gene expression regulation and stress tolerance. The results indicate that miRNAs may play a role in plant stress adaptation by regulating gene expression. The study also highlights the importance of miRNA expression patterns and their targets in understanding the function of these small non-coding RNAs in stress responses. The findings contribute to the understanding of miRNA function in stress conditions and their potential role in plant development and stress tolerance. The study provides a foundation for further research on miRNA function in stress responses and adaptation.This study investigates the role of microRNAs (miRNAs) in stress responses in Arabidopsis thaliana under high-salinity, drought, and cold conditions. Using microarray data, 14 stress-inducible miRNAs were identified, including 10 high-salinity-, 4 drought-, and 10 cold-regulated miRNAs. miR168, miR171, and miR396 responded to all three stresses. RT-PCR analysis showed cross-talk among the signaling pathways of these stresses. Stress-related elements in miRNA promoter regions supported these findings. The study suggests that miRNAs are important regulators in stress responses, with some miRNAs involved in gene expression regulation and stress tolerance. The results indicate that miRNAs may play a role in plant stress adaptation by regulating gene expression. The study also highlights the importance of miRNA expression patterns and their targets in understanding the function of these small non-coding RNAs in stress responses. The findings contribute to the understanding of miRNA function in stress conditions and their potential role in plant development and stress tolerance. The study provides a foundation for further research on miRNA function in stress responses and adaptation.