Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana

Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana

2008 | HAN-HUA LIU, XIN TIAN, YAN-JIE LI, CHANG-AI WU, and CHENG-CHAO ZHENG
This study investigates the expression patterns of microRNAs (miRNAs) in *Arabidopsis thaliana* under high-salinity, drought, and low-temperature stress conditions using microarray analysis. Fourteen stress-inducible miRNAs were identified, including 10 high-salinity-, four drought-, and 10 cold-regulated miRNAs. The expression profiles of these miRNAs were further confirmed by RT-PCR analysis, revealing significant cross-talk among the stress signaling pathways. The presence of stress-related elements in the promoter regions of these miRNAs provides additional evidence supporting their role in stress responses. The findings extend the understanding of miRNAs as regulators of gene expression under stress conditions.This study investigates the expression patterns of microRNAs (miRNAs) in *Arabidopsis thaliana* under high-salinity, drought, and low-temperature stress conditions using microarray analysis. Fourteen stress-inducible miRNAs were identified, including 10 high-salinity-, four drought-, and 10 cold-regulated miRNAs. The expression profiles of these miRNAs were further confirmed by RT-PCR analysis, revealing significant cross-talk among the stress signaling pathways. The presence of stress-related elements in the promoter regions of these miRNAs provides additional evidence supporting their role in stress responses. The findings extend the understanding of miRNAs as regulators of gene expression under stress conditions.
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