Microbial membrane transport proteins (MTPs) are essential for the transport of solutes across cell membranes, playing critical roles in microbial survival, pathogenesis, and antimicrobial resistance. These proteins, including aquaporins and formate-nitrite transporters (FNTs), are transmembrane proteins that facilitate the movement of water and small ions. Recent research has focused on their biotechnological applications, such as the development of biomimetic materials, biosensors, and filtration membranes. Aquaporins, for example, are highly efficient water channels that are abundant in various organisms and are used in water treatment technologies. FNTs, which transport monovalent anions, have been studied for their potential in drug development and bioremediation. MTPs are also important in drug targeting, particularly for pathogens that lack these proteins in their cell membranes. For instance, FNTs in Plasmodium falciparum have been identified as potential targets for malaria treatment. Additionally, MTPs are being explored for their role in improving sensor technologies, such as biosensors that detect specific ions or molecules. Computational methods are being used to predict the functions of transport proteins and to design new ion channels with specific properties. The biotechnological applications of MTPs include the development of desalination membranes, which use biomimetic materials inspired by aquaporins to enhance water transport and solute rejection. These membranes are more energy-efficient compared to traditional methods. Furthermore, MTPs are being used in the production of biofuels and the improvement of microbial strains for industrial applications. The study of MTPs also has implications for gene therapy, as they can be targeted for the treatment of diseases. Overall, the research on MTPs is advancing our understanding of cellular processes and enabling the development of innovative biotechnological applications. The future of MTP research lies in the synthesis of artificial molecules that mimic their functions, leading to more efficient and sustainable technologies.Microbial membrane transport proteins (MTPs) are essential for the transport of solutes across cell membranes, playing critical roles in microbial survival, pathogenesis, and antimicrobial resistance. These proteins, including aquaporins and formate-nitrite transporters (FNTs), are transmembrane proteins that facilitate the movement of water and small ions. Recent research has focused on their biotechnological applications, such as the development of biomimetic materials, biosensors, and filtration membranes. Aquaporins, for example, are highly efficient water channels that are abundant in various organisms and are used in water treatment technologies. FNTs, which transport monovalent anions, have been studied for their potential in drug development and bioremediation. MTPs are also important in drug targeting, particularly for pathogens that lack these proteins in their cell membranes. For instance, FNTs in Plasmodium falciparum have been identified as potential targets for malaria treatment. Additionally, MTPs are being explored for their role in improving sensor technologies, such as biosensors that detect specific ions or molecules. Computational methods are being used to predict the functions of transport proteins and to design new ion channels with specific properties. The biotechnological applications of MTPs include the development of desalination membranes, which use biomimetic materials inspired by aquaporins to enhance water transport and solute rejection. These membranes are more energy-efficient compared to traditional methods. Furthermore, MTPs are being used in the production of biofuels and the improvement of microbial strains for industrial applications. The study of MTPs also has implications for gene therapy, as they can be targeted for the treatment of diseases. Overall, the research on MTPs is advancing our understanding of cellular processes and enabling the development of innovative biotechnological applications. The future of MTP research lies in the synthesis of artificial molecules that mimic their functions, leading to more efficient and sustainable technologies.