This review article focuses on microbial membrane transport proteins (MTPs) and their biotechnological applications. MTPs, which belong to the Major Intrinsic Protein Family, facilitate the transport of hydrophilic solutes across cell membranes. The article highlights the importance of MTPs in microbial survival, pathogenesis, and antimicrobial resistance. It discusses the discovery and characterization of various MTPs, including aquaporins and formate/nitrite transporters, and their roles in different biological processes. The review also explores the potential of MTPs in biotechnological applications such as drug development, biosensors, and bioremediation. Additionally, it covers the use of MTPs in improving metabolic activities, producing industrially important products, and enhancing sensor technologies. The article emphasizes the importance of computational tools and synthetic nanopores in mimicking MTPs for various applications. Finally, it discusses future perspectives and challenges in the field, including the synthesis of artificial molecules that mimic MTP functions.This review article focuses on microbial membrane transport proteins (MTPs) and their biotechnological applications. MTPs, which belong to the Major Intrinsic Protein Family, facilitate the transport of hydrophilic solutes across cell membranes. The article highlights the importance of MTPs in microbial survival, pathogenesis, and antimicrobial resistance. It discusses the discovery and characterization of various MTPs, including aquaporins and formate/nitrite transporters, and their roles in different biological processes. The review also explores the potential of MTPs in biotechnological applications such as drug development, biosensors, and bioremediation. Additionally, it covers the use of MTPs in improving metabolic activities, producing industrially important products, and enhancing sensor technologies. The article emphasizes the importance of computational tools and synthetic nanopores in mimicking MTPs for various applications. Finally, it discusses future perspectives and challenges in the field, including the synthesis of artificial molecules that mimic MTP functions.