Microgels, made from natural or synthetic hydrogel materials, have attracted significant attention as multifunctional cell or drug carriers for tissue engineering and regenerative medicine. They can be aggregated into microporous scaffolds, promoting cell infiltration and proliferation for tissue repair. This review summarizes recent developments in microgel fabrication techniques and applications, discussing various strategies such as emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, and 3D bioprinting. The characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery are elaborated, emphasizing their advantages in cell therapy. The review also discusses ongoing and foreseeable applications and current limitations of microgels in biomedical engineering, highlighting the potential for expanding their use in cell delivery techniques. Microgels are fabricated using various methods, including emulsification, microfluidic techniques, lithography, microfluidic electrospray, centrifugation, and gas-shearing. Each method has its advantages and limitations, with considerations for biocompatibility, monodispersity, and cell viability. For example, emulsification is a common method for generating microgels, while microfluidic techniques allow precise control over microgel morphology and size. Lithography enables the creation of microgels with complex structures, and 3D bioprinting allows the fabrication of microgels with intricate geometries. The review also discusses the use of microgels in cell delivery, highlighting their ability to protect cells during encapsulation and delivery, enhance nutrient and metabolic product exchange, and provide a suitable microenvironment for cell growth and function. The review emphasizes the importance of controlling microgel size and morphology to ensure effective cell delivery and tissue regeneration. Overall, microgels offer a promising platform for cell delivery in tissue engineering and regenerative medicine, with ongoing research aimed at improving their biocompatibility, functionality, and application potential.Microgels, made from natural or synthetic hydrogel materials, have attracted significant attention as multifunctional cell or drug carriers for tissue engineering and regenerative medicine. They can be aggregated into microporous scaffolds, promoting cell infiltration and proliferation for tissue repair. This review summarizes recent developments in microgel fabrication techniques and applications, discussing various strategies such as emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, and 3D bioprinting. The characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery are elaborated, emphasizing their advantages in cell therapy. The review also discusses ongoing and foreseeable applications and current limitations of microgels in biomedical engineering, highlighting the potential for expanding their use in cell delivery techniques. Microgels are fabricated using various methods, including emulsification, microfluidic techniques, lithography, microfluidic electrospray, centrifugation, and gas-shearing. Each method has its advantages and limitations, with considerations for biocompatibility, monodispersity, and cell viability. For example, emulsification is a common method for generating microgels, while microfluidic techniques allow precise control over microgel morphology and size. Lithography enables the creation of microgels with complex structures, and 3D bioprinting allows the fabrication of microgels with intricate geometries. The review also discusses the use of microgels in cell delivery, highlighting their ability to protect cells during encapsulation and delivery, enhance nutrient and metabolic product exchange, and provide a suitable microenvironment for cell growth and function. The review emphasizes the importance of controlling microgel size and morphology to ensure effective cell delivery and tissue regeneration. Overall, microgels offer a promising platform for cell delivery in tissue engineering and regenerative medicine, with ongoing research aimed at improving their biocompatibility, functionality, and application potential.