This review provides a comprehensive overview of recent advancements in the preparation and application of microgels, which are multifunctional materials used in cell delivery and tissue engineering. Microgels, prepared from natural or synthetic hydrogel materials, offer advantages such as high water content, biocompatibility, and degradability, making them suitable for various biomedical applications. The review discusses various fabrication techniques, including emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, and 3D bioprinting, each with its unique characteristics and suitability for different applications. Key highlights include: - The characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery, emphasizing their benefits in cell therapy. - Ongoing and foreseeable applications and current limitations of microgels in biomedical engineering. - Innovative ideas to expand the use of microgels in cell delivery techniques. The review also explores the challenges and future perspectives in the fabrication of cell-laden microgels, highlighting the importance of biocompatibility, monodispersity, and size control. It discusses the potential of microgels in tissue engineering, regenerative medicine, and drug delivery, emphasizing their role in promoting cell growth, differentiation, and tissue repair. The review concludes by outlining new avenues for advancing microgel-based technologies in multidisciplinary biomedical research.This review provides a comprehensive overview of recent advancements in the preparation and application of microgels, which are multifunctional materials used in cell delivery and tissue engineering. Microgels, prepared from natural or synthetic hydrogel materials, offer advantages such as high water content, biocompatibility, and degradability, making them suitable for various biomedical applications. The review discusses various fabrication techniques, including emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, and 3D bioprinting, each with its unique characteristics and suitability for different applications. Key highlights include: - The characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery, emphasizing their benefits in cell therapy. - Ongoing and foreseeable applications and current limitations of microgels in biomedical engineering. - Innovative ideas to expand the use of microgels in cell delivery techniques. The review also explores the challenges and future perspectives in the fabrication of cell-laden microgels, highlighting the importance of biocompatibility, monodispersity, and size control. It discusses the potential of microgels in tissue engineering, regenerative medicine, and drug delivery, emphasizing their role in promoting cell growth, differentiation, and tissue repair. The review concludes by outlining new avenues for advancing microgel-based technologies in multidisciplinary biomedical research.