Microneedle sensors have emerged as a promising technology for point-of-care (POC) diagnostics due to their ability to access interstitial fluid (ISF) non-invasively, enabling real-time, accurate, and cost-effective biomarker detection. This review summarizes recent advancements in microneedle sensors, focusing on their applications in POC diagnostics and personalized medicine. Microneedles, categorized into metals, inorganics, polymers, and hydrogels, have been engineered with various materials and fabrication techniques to achieve high sensitivity, biocompatibility, and mechanical flexibility. These sensors integrate electrochemical and optical sensing technologies, enabling the detection of a wide range of biomarkers, including electrolytes, metabolites, nucleic acids, proteins, and drugs. The integration of microneedles into "lab-on-a-microneedle" platforms has facilitated the development of wearable and portable diagnostic devices, which are essential for decentralized healthcare and personalized medicine. The global POC diagnostics market is growing rapidly, with a projected value of $75.5 billion by 2027. Despite their advantages, challenges remain in ensuring long-term stability, accuracy, and biocompatibility of microneedle sensors. The review also discusses various fabrication techniques, including formative, subtractive, and additive manufacturing, which are critical for producing microneedles with tailored geometries and functionalities. Additionally, the review highlights the potential of microneedles in accessing biological matrices such as blood, ISF, cells, and tissues, enabling the detection of biomarkers at the cellular and tissue levels. The development of microneedle-based biosensors, including metal, inorganic, polymer, and hydrogel-based sensors, has shown promising results in detecting various biomarkers, including glucose, electrolytes, and proteins. The integration of microneedles with flexible electronics and wireless data transmission has further enhanced their potential for real-time, continuous monitoring of health parameters. Overall, microneedle sensors represent a significant advancement in POC diagnostics, offering a minimally invasive, cost-effective, and user-friendly solution for personalized healthcare.Microneedle sensors have emerged as a promising technology for point-of-care (POC) diagnostics due to their ability to access interstitial fluid (ISF) non-invasively, enabling real-time, accurate, and cost-effective biomarker detection. This review summarizes recent advancements in microneedle sensors, focusing on their applications in POC diagnostics and personalized medicine. Microneedles, categorized into metals, inorganics, polymers, and hydrogels, have been engineered with various materials and fabrication techniques to achieve high sensitivity, biocompatibility, and mechanical flexibility. These sensors integrate electrochemical and optical sensing technologies, enabling the detection of a wide range of biomarkers, including electrolytes, metabolites, nucleic acids, proteins, and drugs. The integration of microneedles into "lab-on-a-microneedle" platforms has facilitated the development of wearable and portable diagnostic devices, which are essential for decentralized healthcare and personalized medicine. The global POC diagnostics market is growing rapidly, with a projected value of $75.5 billion by 2027. Despite their advantages, challenges remain in ensuring long-term stability, accuracy, and biocompatibility of microneedle sensors. The review also discusses various fabrication techniques, including formative, subtractive, and additive manufacturing, which are critical for producing microneedles with tailored geometries and functionalities. Additionally, the review highlights the potential of microneedles in accessing biological matrices such as blood, ISF, cells, and tissues, enabling the detection of biomarkers at the cellular and tissue levels. The development of microneedle-based biosensors, including metal, inorganic, polymer, and hydrogel-based sensors, has shown promising results in detecting various biomarkers, including glucose, electrolytes, and proteins. The integration of microneedles with flexible electronics and wireless data transmission has further enhanced their potential for real-time, continuous monitoring of health parameters. Overall, microneedle sensors represent a significant advancement in POC diagnostics, offering a minimally invasive, cost-effective, and user-friendly solution for personalized healthcare.