A pilot study investigated the presence of microplastics in drinking water from various aqueous matrices using Raman microspectroscopy. The study analyzed 34 water samples collected from different points in the drinking water chain in central Italy. Microplastics were detected in some samples, with concentrations ranging from less than 2 particles/L to a maximum of 5 ± 1.5 particles/L. The particles were primarily composed of polyethylene, polypropylene, and polyethylene terephthalate, with sizes ranging from 30 to 100 µm. Some samples, such as those from water kiosks, were found to be free of microplastics. The study also found that microplastics were more prevalent in groundwater samples compared to tap water and bottled water. The results showed that microplastics were present in varying concentrations depending on the matrix, with the highest concentrations observed in groundwater samples. The study highlights the importance of monitoring microplastics in drinking water to assess potential exposure and health risks. The method used, Raman microspectroscopy, was found to be effective in detecting and characterizing microplastics in water samples. The study concludes that microplastics in drinking water pose a potential health risk and that further research is needed to fully understand their impact on human health.A pilot study investigated the presence of microplastics in drinking water from various aqueous matrices using Raman microspectroscopy. The study analyzed 34 water samples collected from different points in the drinking water chain in central Italy. Microplastics were detected in some samples, with concentrations ranging from less than 2 particles/L to a maximum of 5 ± 1.5 particles/L. The particles were primarily composed of polyethylene, polypropylene, and polyethylene terephthalate, with sizes ranging from 30 to 100 µm. Some samples, such as those from water kiosks, were found to be free of microplastics. The study also found that microplastics were more prevalent in groundwater samples compared to tap water and bottled water. The results showed that microplastics were present in varying concentrations depending on the matrix, with the highest concentrations observed in groundwater samples. The study highlights the importance of monitoring microplastics in drinking water to assess potential exposure and health risks. The method used, Raman microspectroscopy, was found to be effective in detecting and characterizing microplastics in water samples. The study concludes that microplastics in drinking water pose a potential health risk and that further research is needed to fully understand their impact on human health.