This pilot study investigates the presence and characterization of microplastics in drinking water from different aqueous matrices using Raman microspectroscopy. The study examines 34 samples collected at various points in the drinking water supply chain in central Italy, including groundwater, water kiosks, public drinking fountains, tap water, and bottled water. The results show that not all samples were contaminated with microplastics, with some water kiosk samples being free of such contaminants. The microplastics content ranged from less than 2 particles/L to a maximum of 5 + 1.5 particles/L, with sizes ranging from 30 to 100 μm, and consisted of common polymers such as polyethylene, polypropylene, and polyethylene terephthalate. The study highlights the importance of monitoring and prevention strategies to reduce potential exposure to microplastics in drinking water, as they pose a risk to human health due to their persistence in the gastrointestinal tract and potential for bioaccumulation. The findings suggest that μ-Raman spectroscopy is a suitable method for routine analysis of microplastics in drinking water, providing a rapid and effective tool for assessing consumer exposure.This pilot study investigates the presence and characterization of microplastics in drinking water from different aqueous matrices using Raman microspectroscopy. The study examines 34 samples collected at various points in the drinking water supply chain in central Italy, including groundwater, water kiosks, public drinking fountains, tap water, and bottled water. The results show that not all samples were contaminated with microplastics, with some water kiosk samples being free of such contaminants. The microplastics content ranged from less than 2 particles/L to a maximum of 5 + 1.5 particles/L, with sizes ranging from 30 to 100 μm, and consisted of common polymers such as polyethylene, polypropylene, and polyethylene terephthalate. The study highlights the importance of monitoring and prevention strategies to reduce potential exposure to microplastics in drinking water, as they pose a risk to human health due to their persistence in the gastrointestinal tract and potential for bioaccumulation. The findings suggest that μ-Raman spectroscopy is a suitable method for routine analysis of microplastics in drinking water, providing a rapid and effective tool for assessing consumer exposure.