This study investigates the presence and characteristics of microplastics (MPs) in indoor deposition samples from university classrooms. MPs were identified using optical microscopy, μRaman spectroscopy, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM–EDX). The dominant type of MP found was fibers, primarily attributed to clothing worn by students and teachers. The MPs ranged in size from 120 to 2222 μm, with the majority being polyamide 6, polypropylene, and polyamide 12. The elemental composition of the MPs included carbon, fluorine, silicon, and oxygen. The study highlights the potential for MPs to degrade into nanoplastics, which could pose greater health and environmental risks. The findings indicate that MPs are prevalent in indoor environments, with higher concentrations compared to outdoor environments. The study also emphasizes the need for further research to understand the sources, pathways, and potential health impacts of MPs in indoor spaces. The results suggest that MPs can originate from various sources, including clothing, shoes, and stationery. The study underscores the importance of addressing indoor MP pollution to mitigate its potential health and environmental risks. The research contributes to the understanding of MPs in indoor environments and provides insights for future studies and policy development.This study investigates the presence and characteristics of microplastics (MPs) in indoor deposition samples from university classrooms. MPs were identified using optical microscopy, μRaman spectroscopy, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM–EDX). The dominant type of MP found was fibers, primarily attributed to clothing worn by students and teachers. The MPs ranged in size from 120 to 2222 μm, with the majority being polyamide 6, polypropylene, and polyamide 12. The elemental composition of the MPs included carbon, fluorine, silicon, and oxygen. The study highlights the potential for MPs to degrade into nanoplastics, which could pose greater health and environmental risks. The findings indicate that MPs are prevalent in indoor environments, with higher concentrations compared to outdoor environments. The study also emphasizes the need for further research to understand the sources, pathways, and potential health impacts of MPs in indoor spaces. The results suggest that MPs can originate from various sources, including clothing, shoes, and stationery. The study underscores the importance of addressing indoor MP pollution to mitigate its potential health and environmental risks. The research contributes to the understanding of MPs in indoor environments and provides insights for future studies and policy development.