This study presents a novel strategy for fabricating robust biomedical coatings by mimicking the foot protein of the mussel, Mytilus edulis. The research focuses on the synthesis and characterization of a catechol-based polyamine (CPA) and its derivatives, including polydopamine (PCPA), heparin-grafted PCPA (Hep-PCPA), and surface-modified PCPA coatings with functional groups such as SeCA, DBCO-ABP, and amino/carboxyl groups. The coatings were evaluated for their chemical and mechanical stability, biocompatibility, and ability to promote cell adhesion and prevent thrombosis. The study also assessed the antibacterial properties of the coatings and their performance in vivo and ex vivo. The results demonstrated that the PCPA coatings exhibited excellent stability under various pH and oxidative conditions, maintained good mechanical properties, and supported cell proliferation and adhesion. The coatings also showed effective NO release and antibacterial activity against E. coli and S. epidermidis. The study highlights the potential of mimicking mussel foot proteins for developing versatile and robust biomedical coatings with broad applications in medical devices and implants.This study presents a novel strategy for fabricating robust biomedical coatings by mimicking the foot protein of the mussel, Mytilus edulis. The research focuses on the synthesis and characterization of a catechol-based polyamine (CPA) and its derivatives, including polydopamine (PCPA), heparin-grafted PCPA (Hep-PCPA), and surface-modified PCPA coatings with functional groups such as SeCA, DBCO-ABP, and amino/carboxyl groups. The coatings were evaluated for their chemical and mechanical stability, biocompatibility, and ability to promote cell adhesion and prevent thrombosis. The study also assessed the antibacterial properties of the coatings and their performance in vivo and ex vivo. The results demonstrated that the PCPA coatings exhibited excellent stability under various pH and oxidative conditions, maintained good mechanical properties, and supported cell proliferation and adhesion. The coatings also showed effective NO release and antibacterial activity against E. coli and S. epidermidis. The study highlights the potential of mimicking mussel foot proteins for developing versatile and robust biomedical coatings with broad applications in medical devices and implants.