The article discusses the impact of aging on mesenchymal stromal cells (MSCs) and their potential applications in regenerative medicine. MSCs play a crucial role in tissue regeneration and homeostasis, but their function declines with age, affecting tissue repair and regeneration. Key aspects of MSC aging include reduced proliferation, impaired differentiation, and genomic instability. These changes are influenced by various factors such as reactive oxygen species (ROS), systemic inflammation, and epigenetic modifications. Understanding these changes is essential for optimizing MSC-based therapies, which require high cellular doses and efficient expansion protocols. The article highlights the need for further research to identify specific pathways involved in MSC aging and to develop methods for better isolation, expansion, and characterization of MSC populations. Additionally, it emphasizes the importance of considering the complex interactions between MSCs, their niches, and the aged tissue environment in regenerative medicine applications.The article discusses the impact of aging on mesenchymal stromal cells (MSCs) and their potential applications in regenerative medicine. MSCs play a crucial role in tissue regeneration and homeostasis, but their function declines with age, affecting tissue repair and regeneration. Key aspects of MSC aging include reduced proliferation, impaired differentiation, and genomic instability. These changes are influenced by various factors such as reactive oxygen species (ROS), systemic inflammation, and epigenetic modifications. Understanding these changes is essential for optimizing MSC-based therapies, which require high cellular doses and efficient expansion protocols. The article highlights the need for further research to identify specific pathways involved in MSC aging and to develop methods for better isolation, expansion, and characterization of MSC populations. Additionally, it emphasizes the importance of considering the complex interactions between MSCs, their niches, and the aged tissue environment in regenerative medicine applications.