Silver nanoparticles exhibit antiviral activity against HIV-1 at non-cytotoxic concentrations, acting as virucidal agents or inhibitors of viral entry. They bind to gp120, preventing CD4-dependent virion binding, fusion, and infectivity. Silver nanoparticles inhibit HIV-1 at early stages of replication, including viral entry and post-entry stages of the life cycle. They are effective against various HIV-1 strains, including resistant ones, and do not induce resistance. Silver nanoparticles show broad-spectrum antiviral activity, making them a promising candidate for HIV-1 prevention. Their mode of action involves direct interaction with viral particles, inactivating them and blocking infection. Silver nanoparticles are more effective than silver ions, suggesting their unique properties. They inhibit HIV-1 transmission by inactivating both cell-free and cell-associated virus. Silver nanoparticles are effective against multiple HIV-1 strains, regardless of tropism, and their antiviral activity is not affected by drug resistance mutations. Silver nanoparticles may interact with gp120's CD4-binding region, preventing viral entry. They also inhibit post-entry stages of infection, including reverse transcription and integration. Silver nanoparticles show potential as virucidal agents, effectively inactivating HIV-1 particles. Their antiviral activity is effective at non-cytotoxic concentrations, making them a promising tool for HIV-1 prevention. Further research is needed to fully understand their mechanism of action and safety for use in preventing HIV-1 transmission.Silver nanoparticles exhibit antiviral activity against HIV-1 at non-cytotoxic concentrations, acting as virucidal agents or inhibitors of viral entry. They bind to gp120, preventing CD4-dependent virion binding, fusion, and infectivity. Silver nanoparticles inhibit HIV-1 at early stages of replication, including viral entry and post-entry stages of the life cycle. They are effective against various HIV-1 strains, including resistant ones, and do not induce resistance. Silver nanoparticles show broad-spectrum antiviral activity, making them a promising candidate for HIV-1 prevention. Their mode of action involves direct interaction with viral particles, inactivating them and blocking infection. Silver nanoparticles are more effective than silver ions, suggesting their unique properties. They inhibit HIV-1 transmission by inactivating both cell-free and cell-associated virus. Silver nanoparticles are effective against multiple HIV-1 strains, regardless of tropism, and their antiviral activity is not affected by drug resistance mutations. Silver nanoparticles may interact with gp120's CD4-binding region, preventing viral entry. They also inhibit post-entry stages of infection, including reverse transcription and integration. Silver nanoparticles show potential as virucidal agents, effectively inactivating HIV-1 particles. Their antiviral activity is effective at non-cytotoxic concentrations, making them a promising tool for HIV-1 prevention. Further research is needed to fully understand their mechanism of action and safety for use in preventing HIV-1 transmission.