This review discusses the complex interactions between phytohormones, particularly auxin, cytokinin (CK), and strigolactones (SL), in regulating root system architecture (RSA) in plants. Root architecture is crucial for water uptake, nutrient absorption, and plant-microbe interactions. Recent studies have shown that phytohormones regulate root development through complex signaling pathways and transcription factor (TF) activation. Auxin is a key regulator of root development, influencing cell division, elongation, and differentiation. It interacts with other hormones such as CK and SL, which also play important roles in root development. CK promotes root growth and development, while SL regulates root architecture and development. The review highlights the cross-talk between these hormones and their roles in different stages of root development. It also discusses the importance of understanding these hormonal interactions for improving crop yield and resilience under various environmental conditions. The study emphasizes the need for further research to understand the molecular mechanisms underlying hormonal cross-talk in root development.This review discusses the complex interactions between phytohormones, particularly auxin, cytokinin (CK), and strigolactones (SL), in regulating root system architecture (RSA) in plants. Root architecture is crucial for water uptake, nutrient absorption, and plant-microbe interactions. Recent studies have shown that phytohormones regulate root development through complex signaling pathways and transcription factor (TF) activation. Auxin is a key regulator of root development, influencing cell division, elongation, and differentiation. It interacts with other hormones such as CK and SL, which also play important roles in root development. CK promotes root growth and development, while SL regulates root architecture and development. The review highlights the cross-talk between these hormones and their roles in different stages of root development. It also discusses the importance of understanding these hormonal interactions for improving crop yield and resilience under various environmental conditions. The study emphasizes the need for further research to understand the molecular mechanisms underlying hormonal cross-talk in root development.