The article by John Condeelis and Ralph Weissleder highlights the critical role of imaging in cancer research, clinical trials, and medical practice. It discusses the rapid advancements in high-resolution fluorescent imaging and MR/PET/CT image registration, which have enabled the bridging of physical scales at the single-cell level. The authors emphasize the synergistic relationship between different imaging technologies and new molecular probes, which are crucial for translating basic science insights into clinical applications. They provide an overview of macroscopic and microscopic imaging techniques, including MRI, PET, SPECT, CT, fluorescence imaging, and multiphoton microscopy, and discuss their applications in breast cancer research. The article also covers the development of advanced imaging platforms, such as hybrid imaging systems, and the challenges and solutions in image processing and analysis. Additionally, it explores the use of imaging agents, reporters, and labeling schemes to label and visualize specific molecules, cells, and biological processes in cancer progression and metastasis. The authors conclude by discussing the potential of these imaging technologies to improve understanding and treatment of cancer.The article by John Condeelis and Ralph Weissleder highlights the critical role of imaging in cancer research, clinical trials, and medical practice. It discusses the rapid advancements in high-resolution fluorescent imaging and MR/PET/CT image registration, which have enabled the bridging of physical scales at the single-cell level. The authors emphasize the synergistic relationship between different imaging technologies and new molecular probes, which are crucial for translating basic science insights into clinical applications. They provide an overview of macroscopic and microscopic imaging techniques, including MRI, PET, SPECT, CT, fluorescence imaging, and multiphoton microscopy, and discuss their applications in breast cancer research. The article also covers the development of advanced imaging platforms, such as hybrid imaging systems, and the challenges and solutions in image processing and analysis. Additionally, it explores the use of imaging agents, reporters, and labeling schemes to label and visualize specific molecules, cells, and biological processes in cancer progression and metastasis. The authors conclude by discussing the potential of these imaging technologies to improve understanding and treatment of cancer.