This study investigates the multiple shedding routes of 2019-nCoV in infected patients. The researchers collected samples from 39 patients and 139 patients in two separate investigations at a Wuhan hospital. They found that the virus could be detected in anal swabs and blood, even when it was not detected in oral swabs. Specifically, more anal swab positives were observed in later stages of infection compared to oral swab positives, suggesting the presence of the virus through the oral-fecal route. Additionally, serological tests showed increased viral antibody titers, indicating that serology can improve detection rates. The study concludes that 2019-nCoV may be transmitted through multiple routes, and both molecular and serological tests are necessary for definitive confirmation of infection.This study investigates the multiple shedding routes of 2019-nCoV in infected patients. The researchers collected samples from 39 patients and 139 patients in two separate investigations at a Wuhan hospital. They found that the virus could be detected in anal swabs and blood, even when it was not detected in oral swabs. Specifically, more anal swab positives were observed in later stages of infection compared to oral swab positives, suggesting the presence of the virus through the oral-fecal route. Additionally, serological tests showed increased viral antibody titers, indicating that serology can improve detection rates. The study concludes that 2019-nCoV may be transmitted through multiple routes, and both molecular and serological tests are necessary for definitive confirmation of infection.