Molecularly imprinted polymers (MIPs) are synthetic materials that mimic the binding properties of natural antibodies, offering high specificity, sensitivity, and reusability. This review highlights the advancements and applications of MIPs in biochemistry, biomedicine, and biotechnology. MIPs are particularly useful in drug delivery systems due to their ability to enhance drug loading and controlled release, making them a promising alternative to traditional polymeric systems. They are also used in cell recognition, enzyme applications, and in vivo studies, demonstrating their versatility in various fields. The review discusses the challenges and future prospects of MIPs, emphasizing their potential in personalized medicine and targeted therapies. Despite ongoing research into their long-term toxicity and biocompatibility, MIPs show significant promise as a robust and eco-friendly material for a wide range of applications.Molecularly imprinted polymers (MIPs) are synthetic materials that mimic the binding properties of natural antibodies, offering high specificity, sensitivity, and reusability. This review highlights the advancements and applications of MIPs in biochemistry, biomedicine, and biotechnology. MIPs are particularly useful in drug delivery systems due to their ability to enhance drug loading and controlled release, making them a promising alternative to traditional polymeric systems. They are also used in cell recognition, enzyme applications, and in vivo studies, demonstrating their versatility in various fields. The review discusses the challenges and future prospects of MIPs, emphasizing their potential in personalized medicine and targeted therapies. Despite ongoing research into their long-term toxicity and biocompatibility, MIPs show significant promise as a robust and eco-friendly material for a wide range of applications.