The article provides a comprehensive overview of Molecularly Imprinted Polymers (MIPs) and their applications. MIPs are synthetic materials designed to mimic natural recognition entities like antibodies and biological receptors, offering high selectivity and affinity for specific analytes. The review covers the preparation methods, including the molecular imprinting process, and discusses various applications such as chemical sensing, separation science, drug delivery, and catalysis. Key aspects of MIPs preparation, including prepolymerization studies and optimization of synthesis parameters, are highlighted. The physical forms of MIPs, such as beads, membranes, and monoliths, are also explored. The article emphasizes the importance of solvent choice, cross-linker selection, and monomer properties in achieving optimal MIP performance. Additionally, it addresses critical aspects like the design of water-compatible MIPs and the challenges in achieving uniform particle size distribution. The applications of MIPs in separation techniques, solid-phase extraction (SPE), and immunoassay-type analyses are discussed, along with their potential in therapeutic and medical fields. The review concludes by outlining the future prospects of MIPs in various scientific and industrial applications.The article provides a comprehensive overview of Molecularly Imprinted Polymers (MIPs) and their applications. MIPs are synthetic materials designed to mimic natural recognition entities like antibodies and biological receptors, offering high selectivity and affinity for specific analytes. The review covers the preparation methods, including the molecular imprinting process, and discusses various applications such as chemical sensing, separation science, drug delivery, and catalysis. Key aspects of MIPs preparation, including prepolymerization studies and optimization of synthesis parameters, are highlighted. The physical forms of MIPs, such as beads, membranes, and monoliths, are also explored. The article emphasizes the importance of solvent choice, cross-linker selection, and monomer properties in achieving optimal MIP performance. Additionally, it addresses critical aspects like the design of water-compatible MIPs and the challenges in achieving uniform particle size distribution. The applications of MIPs in separation techniques, solid-phase extraction (SPE), and immunoassay-type analyses are discussed, along with their potential in therapeutic and medical fields. The review concludes by outlining the future prospects of MIPs in various scientific and industrial applications.