The NIAAA (National Institute on Alcohol Abuse and Alcoholism) model of chronic and binge ethanol feeding in mice is described as a method to study alcoholic liver injury. This model involves 10 days of ad libitum feeding with a Lieber-DeCarli ethanol liquid diet followed by a single binge of ethanol (5 g/kg body weight). The protocol induces significant liver injury, inflammation, and fatty liver, mimicking acute-on-chronic alcoholic liver injury in patients. The model can be extended to longer chronic feeding periods (up to 8 weeks) plus single or multiple binges. Key steps include acclimatization to tube feeding, chronic ethanol feeding, and acute ethanol gavage. The model is cost-effective, time-efficient, and applicable to various research areas, including ALD and other organ damage caused by alcohol consumption. The protocol is detailed, including materials, equipment, and procedures, and includes troubleshooting tips. The model has been validated with various outcome measures, such as serum ALT and AST levels, plasma ethanol concentration, and liver histology.The NIAAA (National Institute on Alcohol Abuse and Alcoholism) model of chronic and binge ethanol feeding in mice is described as a method to study alcoholic liver injury. This model involves 10 days of ad libitum feeding with a Lieber-DeCarli ethanol liquid diet followed by a single binge of ethanol (5 g/kg body weight). The protocol induces significant liver injury, inflammation, and fatty liver, mimicking acute-on-chronic alcoholic liver injury in patients. The model can be extended to longer chronic feeding periods (up to 8 weeks) plus single or multiple binges. Key steps include acclimatization to tube feeding, chronic ethanol feeding, and acute ethanol gavage. The model is cost-effective, time-efficient, and applicable to various research areas, including ALD and other organ damage caused by alcohol consumption. The protocol is detailed, including materials, equipment, and procedures, and includes troubleshooting tips. The model has been validated with various outcome measures, such as serum ALT and AST levels, plasma ethanol concentration, and liver histology.