Mouse oocytes store protein aggregates in degradative super-organelles called endolysosomal vesicular assemblies (ELVAs). These structures, composed of endolysosomes, autophagosomes, and proteasomes, sequester aggregated proteins in immature oocytes and degrade them upon maturation to support healthy embryogenesis. Failure to degrade these aggregates leads to early embryonic arrest. ELVAs are non-membrane-bound compartments held together by a protein matrix, primarily RUFY1, which facilitates the assembly and stability of these organelles. During oocyte maturation, ELVAs relocate to the cortex in an actin-dependent manner and subsequently undergo exocytosis, releasing their contents to the embryo. This process is crucial for clearing toxic protein aggregates and ensuring proper embryonic development. The degradation activity within ELVAs increases during maturation, allowing the oocyte to efficiently remove harmful aggregates. The study highlights the importance of ELVAs in maintaining proteostasis in long-lived cells, ensuring the transmission of a healthy cytoplasm to the next generation.Mouse oocytes store protein aggregates in degradative super-organelles called endolysosomal vesicular assemblies (ELVAs). These structures, composed of endolysosomes, autophagosomes, and proteasomes, sequester aggregated proteins in immature oocytes and degrade them upon maturation to support healthy embryogenesis. Failure to degrade these aggregates leads to early embryonic arrest. ELVAs are non-membrane-bound compartments held together by a protein matrix, primarily RUFY1, which facilitates the assembly and stability of these organelles. During oocyte maturation, ELVAs relocate to the cortex in an actin-dependent manner and subsequently undergo exocytosis, releasing their contents to the embryo. This process is crucial for clearing toxic protein aggregates and ensuring proper embryonic development. The degradation activity within ELVAs increases during maturation, allowing the oocyte to efficiently remove harmful aggregates. The study highlights the importance of ELVAs in maintaining proteostasis in long-lived cells, ensuring the transmission of a healthy cytoplasm to the next generation.