This study presents a novel approach to treating Methicillin-resistant *Staphylococcus aureus* (MRSA)-infected wounds using multifunctional microneedle patches (MNs) loaded with zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) encapsulating low molecular weight fucoidan (Fu) and coated with hyaluronic acid (HA). The HAZ@Fu NPs were designed to target MRSA at the subcellular level, particularly within lysosomes, and to promote wound healing. The MNs, loaded with HAZ@Fu NPs, were fabricated using a photocrosslinked gelatin methacryloyl (GelMA) hydrogel and applied to MRSA-infected full-thickness cutaneous wounds in mice. In vitro and in vivo studies demonstrated that the MNs effectively delivered HAZ@Fu NPs deep into the dermal layers, targeting MRSA and releasing their components sustainably. The zinc ions, Fu, and HA contributed to antibacterial activity, reduced inflammation, promoted epithelial regeneration, and enhanced neovascularization. RNA sequencing and KEGG pathway analysis revealed that the PI3K-Akt pathway played a crucial role in facilitating wound healing. The results indicate that the synergistic application of HAZ@Fu NPs with biodegradable MNs holds significant potential for treating infected wounds.This study presents a novel approach to treating Methicillin-resistant *Staphylococcus aureus* (MRSA)-infected wounds using multifunctional microneedle patches (MNs) loaded with zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) encapsulating low molecular weight fucoidan (Fu) and coated with hyaluronic acid (HA). The HAZ@Fu NPs were designed to target MRSA at the subcellular level, particularly within lysosomes, and to promote wound healing. The MNs, loaded with HAZ@Fu NPs, were fabricated using a photocrosslinked gelatin methacryloyl (GelMA) hydrogel and applied to MRSA-infected full-thickness cutaneous wounds in mice. In vitro and in vivo studies demonstrated that the MNs effectively delivered HAZ@Fu NPs deep into the dermal layers, targeting MRSA and releasing their components sustainably. The zinc ions, Fu, and HA contributed to antibacterial activity, reduced inflammation, promoted epithelial regeneration, and enhanced neovascularization. RNA sequencing and KEGG pathway analysis revealed that the PI3K-Akt pathway played a crucial role in facilitating wound healing. The results indicate that the synergistic application of HAZ@Fu NPs with biodegradable MNs holds significant potential for treating infected wounds.