This study presents a method for multiplexed 3D super-resolution imaging using DNA-PAINT and Exchange-PAINT. DNA-PAINT uses fluorescently labeled oligonucleotides to achieve sub-10 nm spatial resolution in vitro, enabling the imaging of synthetic DNA structures and proteins in fixed cells. The method relies on the transient binding of fluorescently labeled oligonucleotides to complementary docking strands, allowing for high-resolution imaging without the need for specialized equipment. Exchange-PAINT extends this approach by enabling sequential imaging of multiple targets using a single dye and laser, achieving ten-color super-resolution imaging of synthetic DNA structures and four-color imaging of proteins in fixed cells. The study demonstrates the ability to image multiple targets in fixed cells with high specificity and resolution, and shows that Exchange-PAINT can be used for 3D imaging of cellular components. The method is simple, scalable, and does not require complex optical setups or sample preparation. The results show that DNA-PAINT and Exchange-PAINT can achieve high-resolution imaging of cellular structures, with minimal sample distortion and no crosstalk between color channels. The study also highlights the potential of these methods for further development in the study of complex biomolecular systems.This study presents a method for multiplexed 3D super-resolution imaging using DNA-PAINT and Exchange-PAINT. DNA-PAINT uses fluorescently labeled oligonucleotides to achieve sub-10 nm spatial resolution in vitro, enabling the imaging of synthetic DNA structures and proteins in fixed cells. The method relies on the transient binding of fluorescently labeled oligonucleotides to complementary docking strands, allowing for high-resolution imaging without the need for specialized equipment. Exchange-PAINT extends this approach by enabling sequential imaging of multiple targets using a single dye and laser, achieving ten-color super-resolution imaging of synthetic DNA structures and four-color imaging of proteins in fixed cells. The study demonstrates the ability to image multiple targets in fixed cells with high specificity and resolution, and shows that Exchange-PAINT can be used for 3D imaging of cellular components. The method is simple, scalable, and does not require complex optical setups or sample preparation. The results show that DNA-PAINT and Exchange-PAINT can achieve high-resolution imaging of cellular structures, with minimal sample distortion and no crosstalk between color channels. The study also highlights the potential of these methods for further development in the study of complex biomolecular systems.