The MycoCosm portal, developed by the US Department of Energy Joint Genome Institute, is a comprehensive fungal genomics resource that supports the integration, analysis, and dissemination of fungal genome sequences and other 'omics' data. It provides interactive web-based tools for in-depth multidimensional analysis of individual genomes and efficient comparative genomics of fungi. Since its inception, MycoCosm has grown to host over 250 publicly available fungal genomes, with 80% sequenced by JGI and 20% by other groups for comparative purposes. The portal includes flexible tools for analyzing large datasets and extends user community participation to the entire genome project cycle, from species nomination for sequencing to genome annotation and comparative genomics. MycoCosm also features uniform genome annotation using the JGI Annotation Pipeline, which integrates various tools for gene prediction, annotation, and analysis. Additionally, it offers tools for grouping genomes for comparative analyses, including PhyloGroups and EcoGroups, to facilitate the exploration of genetic and ecological similarities among fungi. The portal encourages user engagement through the 1000 Fungal Genomes Project, where users can nominate new species for sequencing, contributing to the comprehensive understanding of the Fungal Tree of Life. Future plans include developing new interactive tools for transcriptomics, epigenomics, population genomics, and metagenomics to support data analysis and hypothesis development.The MycoCosm portal, developed by the US Department of Energy Joint Genome Institute, is a comprehensive fungal genomics resource that supports the integration, analysis, and dissemination of fungal genome sequences and other 'omics' data. It provides interactive web-based tools for in-depth multidimensional analysis of individual genomes and efficient comparative genomics of fungi. Since its inception, MycoCosm has grown to host over 250 publicly available fungal genomes, with 80% sequenced by JGI and 20% by other groups for comparative purposes. The portal includes flexible tools for analyzing large datasets and extends user community participation to the entire genome project cycle, from species nomination for sequencing to genome annotation and comparative genomics. MycoCosm also features uniform genome annotation using the JGI Annotation Pipeline, which integrates various tools for gene prediction, annotation, and analysis. Additionally, it offers tools for grouping genomes for comparative analyses, including PhyloGroups and EcoGroups, to facilitate the exploration of genetic and ecological similarities among fungi. The portal encourages user engagement through the 1000 Fungal Genomes Project, where users can nominate new species for sequencing, contributing to the comprehensive understanding of the Fungal Tree of Life. Future plans include developing new interactive tools for transcriptomics, epigenomics, population genomics, and metagenomics to support data analysis and hypothesis development.