Nanobiomaterials and bioinks are crucial in 3D bioprinting for tissue engineering and artificial organs. Bioinks combine live cells and biomaterials, often with tissue factors, to create structures that mimic human tissues. Bioprinting uses biomaterials or bioinks to produce 3D constructs with properties similar to natural tissues. These structures are used in tissue engineering for healing and in vitro testing of drugs and vaccines. The challenge lies in ensuring physicochemical and biological signals regulate cell activity. Nano-biomaterials can control cell fate, aiding differentiation and biofabrication. Nano-composite bioinks enable instructive scaffolds and respond to external stimuli, enhancing healthcare applications. The study emphasizes the potential of nano-biomaterials in bioprinting for tissue regeneration. Bioinks are created from polymers, which can support tissue regeneration and cell processes. They are used in bioprinting to create scaffolds or constructs for cell transplantation. Bioprinting involves layer-by-layer deposition of biodegradable polymers or solutions. Live cell printing is called bioprinting, while non-living cell printing is called printing. Extrusion bioprinting is a popular method using mechanical forces. Three types of mechanical forces are used: screw-driven, piston-driven, and pneumatic. Pneumatic-driven printing is popular due to its simplicity and low cost. Screw-driven and piston-driven methods offer higher mechanical forces and better control over bioink flow. Nanobio composite-based inks are used to integrate biomolecules into bioprinted structures. These inks can release growth factors, enhancing cell differentiation. For example, TGF-β1 nano-carriers in bioinks improve MSC chondrogenesis. The 3D-Bloodprinting technique uses stereolithography to create cartilage constructs. The bioink contains GelMA and TGF-β1-loaded nano-spheres. The nano-spheres degrade slowly, releasing TGF-β1. qPCR studies show increased collagen II and collagen I expression in MSCs. This method allows for dynamic biomolecule presence in the ECM. Remote-controlled nanobiocomposite inks can manipulate nanoparticles through external stimuli. Magnetic fields can organize superparamagnetic nanoparticles, enabling further patterning. Iron oxide nanoparticles in alginate solutions affect cell viability and bioprinting parameters. Post-bioprinting, magnets can move and accumulate nanoparticles, allowing for cell manipulation. However, magnetic manipulation may alter the printed structure's fidelity and displace non-targeted materials. Nanobioinks are used to create artificial tissues with functional properties. Nanomaterials like liposomes, QDs, CNTs, and MSNs are promising for cancer therapy. These materials can be integrated into bioprinted structures to create active biological constructs. For example, hydrogels with cells and nanoelectronic components can mimic human heart tissue. Bionic tissues can senseNanobiomaterials and bioinks are crucial in 3D bioprinting for tissue engineering and artificial organs. Bioinks combine live cells and biomaterials, often with tissue factors, to create structures that mimic human tissues. Bioprinting uses biomaterials or bioinks to produce 3D constructs with properties similar to natural tissues. These structures are used in tissue engineering for healing and in vitro testing of drugs and vaccines. The challenge lies in ensuring physicochemical and biological signals regulate cell activity. Nano-biomaterials can control cell fate, aiding differentiation and biofabrication. Nano-composite bioinks enable instructive scaffolds and respond to external stimuli, enhancing healthcare applications. The study emphasizes the potential of nano-biomaterials in bioprinting for tissue regeneration. Bioinks are created from polymers, which can support tissue regeneration and cell processes. They are used in bioprinting to create scaffolds or constructs for cell transplantation. Bioprinting involves layer-by-layer deposition of biodegradable polymers or solutions. Live cell printing is called bioprinting, while non-living cell printing is called printing. Extrusion bioprinting is a popular method using mechanical forces. Three types of mechanical forces are used: screw-driven, piston-driven, and pneumatic. Pneumatic-driven printing is popular due to its simplicity and low cost. Screw-driven and piston-driven methods offer higher mechanical forces and better control over bioink flow. Nanobio composite-based inks are used to integrate biomolecules into bioprinted structures. These inks can release growth factors, enhancing cell differentiation. For example, TGF-β1 nano-carriers in bioinks improve MSC chondrogenesis. The 3D-Bloodprinting technique uses stereolithography to create cartilage constructs. The bioink contains GelMA and TGF-β1-loaded nano-spheres. The nano-spheres degrade slowly, releasing TGF-β1. qPCR studies show increased collagen II and collagen I expression in MSCs. This method allows for dynamic biomolecule presence in the ECM. Remote-controlled nanobiocomposite inks can manipulate nanoparticles through external stimuli. Magnetic fields can organize superparamagnetic nanoparticles, enabling further patterning. Iron oxide nanoparticles in alginate solutions affect cell viability and bioprinting parameters. Post-bioprinting, magnets can move and accumulate nanoparticles, allowing for cell manipulation. However, magnetic manipulation may alter the printed structure's fidelity and displace non-targeted materials. Nanobioinks are used to create artificial tissues with functional properties. Nanomaterials like liposomes, QDs, CNTs, and MSNs are promising for cancer therapy. These materials can be integrated into bioprinted structures to create active biological constructs. For example, hydrogels with cells and nanoelectronic components can mimic human heart tissue. Bionic tissues can sense