This study presents a smartphone-based electrochemical microfluidic device integrated with nanobody recognition for the detection of C-reactive protein (CRP), an inflammation biomarker. The device combines a flow-through microfluidic system with a near-field communication (NFC) potentiostat, enabling automated sequential flow and real-time data analysis on a smartphone. The system uses nanobodies, which are small, recombinant proteins that offer advantages over traditional IgG antibodies in terms of sensitivity, selectivity, and storage stability. The device was tested with three biological samples (artificial serum, plasma, and whole blood) and demonstrated high accuracy and reliability in CRP detection. The sensor is portable, cost-effective, and capable of rapid analysis within 15 minutes. It was compared with a commercial potentiostat (PalmSens4) and showed good agreement in CRP quantification. The device also exhibited excellent storage stability under various conditions. The results indicate that the NFC-based system is a promising tool for point-of-care testing (POCT) due to its simplicity, low cost, and portability. The device can be adapted for the detection of other soluble biomarkers by replacing the nanobody recognition element. The study highlights the potential of integrating nanobodies with microfluidic and NFC technologies for the development of advanced diagnostic tools.This study presents a smartphone-based electrochemical microfluidic device integrated with nanobody recognition for the detection of C-reactive protein (CRP), an inflammation biomarker. The device combines a flow-through microfluidic system with a near-field communication (NFC) potentiostat, enabling automated sequential flow and real-time data analysis on a smartphone. The system uses nanobodies, which are small, recombinant proteins that offer advantages over traditional IgG antibodies in terms of sensitivity, selectivity, and storage stability. The device was tested with three biological samples (artificial serum, plasma, and whole blood) and demonstrated high accuracy and reliability in CRP detection. The sensor is portable, cost-effective, and capable of rapid analysis within 15 minutes. It was compared with a commercial potentiostat (PalmSens4) and showed good agreement in CRP quantification. The device also exhibited excellent storage stability under various conditions. The results indicate that the NFC-based system is a promising tool for point-of-care testing (POCT) due to its simplicity, low cost, and portability. The device can be adapted for the detection of other soluble biomarkers by replacing the nanobody recognition element. The study highlights the potential of integrating nanobodies with microfluidic and NFC technologies for the development of advanced diagnostic tools.