This review discusses the use of nanomaterials for co-immobilizing multiple enzymes to enhance synergistic enzyme activity and catalytic efficiency. Nanomaterials, such as nanoparticles, nanowires, nanofilms, and nanoporous materials, offer advantages like tunable morphology, high specific surface area, and abundant chemically active sites, which significantly improve enzyme stability, activity, and catalytic efficiency. The review outlines common methods and strategies for enzyme co-immobilization, including physical and chemical approaches, and highlights recent research advances in nanomaterials for this purpose over the past five years. It also discusses the advantages and challenges of these nanomaterials and explores potential future directions. The text is divided into sections covering different types of nanocarriers, including metal-based nanoparticles, magnetic nanoparticles, metal-organic frameworks (MOFs), mesoporous silica nanoparticles, carbon-based nanomaterials, and organic-inorganic hybrid nanomaterials. Each section provides examples of specific enzymes co-immobilized using these nanomaterials and their applications in various fields such as biocatalysis, biosensing, and medical diagnostics.This review discusses the use of nanomaterials for co-immobilizing multiple enzymes to enhance synergistic enzyme activity and catalytic efficiency. Nanomaterials, such as nanoparticles, nanowires, nanofilms, and nanoporous materials, offer advantages like tunable morphology, high specific surface area, and abundant chemically active sites, which significantly improve enzyme stability, activity, and catalytic efficiency. The review outlines common methods and strategies for enzyme co-immobilization, including physical and chemical approaches, and highlights recent research advances in nanomaterials for this purpose over the past five years. It also discusses the advantages and challenges of these nanomaterials and explores potential future directions. The text is divided into sections covering different types of nanocarriers, including metal-based nanoparticles, magnetic nanoparticles, metal-organic frameworks (MOFs), mesoporous silica nanoparticles, carbon-based nanomaterials, and organic-inorganic hybrid nanomaterials. Each section provides examples of specific enzymes co-immobilized using these nanomaterials and their applications in various fields such as biocatalysis, biosensing, and medical diagnostics.