Nanoparticle-based strategies for managing biofilm infections in wounds are reviewed, highlighting four main types of nanoparticle approaches: metallic nanoparticles (e.g., silver, copper, gold), phototherapy-based nanoparticles, nanoparticles targeting extracellular polymeric substances (EPSs), and nanoparticles inducing biofilm dispersion. These strategies aim to enhance treatment outcomes and reduce antibiotic use. Biofilms, complex bacterial communities, are resistant to immune responses and traditional treatments, posing challenges in wound healing. Wound dressings, a key treatment avenue, are increasingly incorporating nanoparticles with antimicrobial properties. Metallic nanoparticles disrupt bacterial cell membranes and induce oxidative stress, while phototherapy-based nanoparticles generate reactive oxygen species or heat to destroy microbes. Nanoparticles targeting EPSs enhance antimicrobial agent penetration, and those inducing biofilm dispersion weaken biofilm defenses. Despite their potential, challenges include nanoparticle toxicity, size, and the need for targeted delivery. Silver, copper, and gold nanoparticles show promise but face issues like cytotoxicity and bacterial resistance. Polymer nanoparticles, such as chitosan, offer biocompatibility and antimicrobial activity. Metal-organic frameworks (MOFs) and 2D nanosheets also show potential in disrupting biofilms. Photodynamic and photothermal therapies use light to generate reactive oxygen species or heat, respectively, to kill bacteria. Challenges include ensuring biosafety, scalability, and environmental impact. Overall, nanoparticle-based strategies offer innovative solutions for managing biofilm infections, reducing antibiotic use, and improving wound healing.Nanoparticle-based strategies for managing biofilm infections in wounds are reviewed, highlighting four main types of nanoparticle approaches: metallic nanoparticles (e.g., silver, copper, gold), phototherapy-based nanoparticles, nanoparticles targeting extracellular polymeric substances (EPSs), and nanoparticles inducing biofilm dispersion. These strategies aim to enhance treatment outcomes and reduce antibiotic use. Biofilms, complex bacterial communities, are resistant to immune responses and traditional treatments, posing challenges in wound healing. Wound dressings, a key treatment avenue, are increasingly incorporating nanoparticles with antimicrobial properties. Metallic nanoparticles disrupt bacterial cell membranes and induce oxidative stress, while phototherapy-based nanoparticles generate reactive oxygen species or heat to destroy microbes. Nanoparticles targeting EPSs enhance antimicrobial agent penetration, and those inducing biofilm dispersion weaken biofilm defenses. Despite their potential, challenges include nanoparticle toxicity, size, and the need for targeted delivery. Silver, copper, and gold nanoparticles show promise but face issues like cytotoxicity and bacterial resistance. Polymer nanoparticles, such as chitosan, offer biocompatibility and antimicrobial activity. Metal-organic frameworks (MOFs) and 2D nanosheets also show potential in disrupting biofilms. Photodynamic and photothermal therapies use light to generate reactive oxygen species or heat, respectively, to kill bacteria. Challenges include ensuring biosafety, scalability, and environmental impact. Overall, nanoparticle-based strategies offer innovative solutions for managing biofilm infections, reducing antibiotic use, and improving wound healing.