This review discusses the use of nanoparticles in managing biofilm infections in wounds, categorizing nanoparticle-based approaches into four main types: metallic nanoparticles, phototherapy-based nanoparticles, nanoparticles that disrupt extracellular polymeric substances (EPS), and nanoparticles that induce biofilm dispersion. Metallic nanoparticles, such as silver and copper, disrupt bacterial cell membranes and induce oxidative stress. Phototherapy-based nanoparticles, including photodynamic and photothermal therapy, target and destroy bacteria by generating reactive oxygen species or localized heat, respectively. Nanoparticles designed to disrupt EPS enhance the penetration and efficacy of antimicrobial agents. Nanoparticles that induce biofilm dispersion weaken the biofilm's defense and restore susceptibility to antimicrobials. Each method has unique advantages and challenges, and the selection of an appropriate treatment depends on the specific wound environment and biofilm type. The integration of these nanoparticles into wound dressings offers enhanced treatment outcomes and reduces antibiotic usage, aligning with the need for innovative solutions in the fight against antibiotic-resistant infections.This review discusses the use of nanoparticles in managing biofilm infections in wounds, categorizing nanoparticle-based approaches into four main types: metallic nanoparticles, phototherapy-based nanoparticles, nanoparticles that disrupt extracellular polymeric substances (EPS), and nanoparticles that induce biofilm dispersion. Metallic nanoparticles, such as silver and copper, disrupt bacterial cell membranes and induce oxidative stress. Phototherapy-based nanoparticles, including photodynamic and photothermal therapy, target and destroy bacteria by generating reactive oxygen species or localized heat, respectively. Nanoparticles designed to disrupt EPS enhance the penetration and efficacy of antimicrobial agents. Nanoparticles that induce biofilm dispersion weaken the biofilm's defense and restore susceptibility to antimicrobials. Each method has unique advantages and challenges, and the selection of an appropriate treatment depends on the specific wound environment and biofilm type. The integration of these nanoparticles into wound dressings offers enhanced treatment outcomes and reduces antibiotic usage, aligning with the need for innovative solutions in the fight against antibiotic-resistant infections.