This review discusses nanotechnological strategies for engineering complex tissues. Tissue engineering aims to develop functional substitutes for damaged tissues and organs by seeding cells on biomaterial scaffolds that mimic the extracellular matrix (ECM). The ECM is a nanocomposite with a complex structure that regulates essential cellular functions. Recent advances in nanotechnology have enabled the design of advanced nanocomposite scaffolds that better mimic the ECM, allowing for the assembly of more complex and larger functional tissues. The review highlights the importance of the ECM's nanoscale structure in promoting tissue organization and discusses the impact of nanostructures on scaffold properties and their use in monitoring engineered tissues. It also examines various nanodevices used to trigger tissue development processes and outlines the challenges and prospects of applying nanotechnology in tissue engineering. Key challenges include creating 3D porous scaffolds with nanotopographies, ensuring biocompatibility and biodegradation of nanomaterials, and developing nanotechnological tools to control and guide cells to desired locations in 3D matrices. The review also discusses the potential of nanomaterials such as carbon nanotubes and nanowires in improving mechanical properties, electrical conductivity, and cell adhesion. Nanotechnology has the potential to significantly advance therapeutic methods based on tissue engineering. Future strategies may involve incorporating intelligent nanoscale biosensors inside scaffolds to monitor tissue development and trigger the release of compensating cues. Additionally, smart nanoparticulate systems could be used to recruit stem cells to desired sites in the body and instruct tissue formation in vivo. The review concludes with a discussion of the challenges and prospects of applying nanotechnology in tissue engineering, emphasizing the need for further research and development.This review discusses nanotechnological strategies for engineering complex tissues. Tissue engineering aims to develop functional substitutes for damaged tissues and organs by seeding cells on biomaterial scaffolds that mimic the extracellular matrix (ECM). The ECM is a nanocomposite with a complex structure that regulates essential cellular functions. Recent advances in nanotechnology have enabled the design of advanced nanocomposite scaffolds that better mimic the ECM, allowing for the assembly of more complex and larger functional tissues. The review highlights the importance of the ECM's nanoscale structure in promoting tissue organization and discusses the impact of nanostructures on scaffold properties and their use in monitoring engineered tissues. It also examines various nanodevices used to trigger tissue development processes and outlines the challenges and prospects of applying nanotechnology in tissue engineering. Key challenges include creating 3D porous scaffolds with nanotopographies, ensuring biocompatibility and biodegradation of nanomaterials, and developing nanotechnological tools to control and guide cells to desired locations in 3D matrices. The review also discusses the potential of nanomaterials such as carbon nanotubes and nanowires in improving mechanical properties, electrical conductivity, and cell adhesion. Nanotechnology has the potential to significantly advance therapeutic methods based on tissue engineering. Future strategies may involve incorporating intelligent nanoscale biosensors inside scaffolds to monitor tissue development and trigger the release of compensating cues. Additionally, smart nanoparticulate systems could be used to recruit stem cells to desired sites in the body and instruct tissue formation in vivo. The review concludes with a discussion of the challenges and prospects of applying nanotechnology in tissue engineering, emphasizing the need for further research and development.