This study explores the use of decellularized extracellular matrix (dECM) hydrogels derived from porcine and human renal cortex to enhance kidney organoid generation and promote in vitro angiogenesis. The dECM hydrogels are fabricated using a decellularization protocol that preserves the structural and biochemical properties of the ECM. These hydrogels are then used in combination with human pluripotent stem cell (hPSC)-derived renal progenitor cells to define new approaches for 2D and 3D kidney organoid differentiation. The results show that the presence of these biomaterials facilitates renal differentiation and the formation of an endogenous vascular component in kidney organoids. A novel method for producing kidney organoids with vascular-like structures is developed by assembling hPSC-derived endothelial-like organoids with kidney organoids in 3D. The study also demonstrates the angiogenic potential of these biomaterials through transplantation in the chick chorioallantoic membrane (CAM). Overall, this work highlights the importance of cell-to-ECM interactions during kidney organoid derivation and provides insights into kidney organoid morphogenesis and ECM interactions, offering potential applications in personalized medicine and disease modeling.This study explores the use of decellularized extracellular matrix (dECM) hydrogels derived from porcine and human renal cortex to enhance kidney organoid generation and promote in vitro angiogenesis. The dECM hydrogels are fabricated using a decellularization protocol that preserves the structural and biochemical properties of the ECM. These hydrogels are then used in combination with human pluripotent stem cell (hPSC)-derived renal progenitor cells to define new approaches for 2D and 3D kidney organoid differentiation. The results show that the presence of these biomaterials facilitates renal differentiation and the formation of an endogenous vascular component in kidney organoids. A novel method for producing kidney organoids with vascular-like structures is developed by assembling hPSC-derived endothelial-like organoids with kidney organoids in 3D. The study also demonstrates the angiogenic potential of these biomaterials through transplantation in the chick chorioallantoic membrane (CAM). Overall, this work highlights the importance of cell-to-ECM interactions during kidney organoid derivation and provides insights into kidney organoid morphogenesis and ECM interactions, offering potential applications in personalized medicine and disease modeling.