The National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) database is a curated, non-redundant collection of genomic, transcriptomic, and proteomic sequences. It aims to provide comprehensive and up-to-date sequence information for various species, including over 2400 organisms and more than one million proteins. RefSeq integrates data from GenBank and other sources, ensuring accurate and current annotations. The database is accessible via multiple interfaces, including Entrez queries, BLAST, FTP, and links within other NCBI resources. RefSeq records are annotated with coding regions, conserved domains, variations, references, and other features, using a combination of automated and manual curation. The database is regularly updated to incorporate new genome assemblies and is used internationally as a standard for genome annotation.The National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) database is a curated, non-redundant collection of genomic, transcriptomic, and proteomic sequences. It aims to provide comprehensive and up-to-date sequence information for various species, including over 2400 organisms and more than one million proteins. RefSeq integrates data from GenBank and other sources, ensuring accurate and current annotations. The database is accessible via multiple interfaces, including Entrez queries, BLAST, FTP, and links within other NCBI resources. RefSeq records are annotated with coding regions, conserved domains, variations, references, and other features, using a combination of automated and manual curation. The database is regularly updated to incorporate new genome assemblies and is used internationally as a standard for genome annotation.