This study introduces a DNA hydrogel (DNAgel) inspired by neutrophil extracellular traps (NETs) to address severe traumatic bleeding. DNAgel is a natural nucleic acid-based material with rapid water absorption, high swelling, and strong tissue adhesion, effectively stopping bleeding. It fills the defect cavity, exerts pressure on bleeding vessels, and promotes coagulation through an artificial DNA scaffold for erythrocyte adhesion and platelet activation. In male rat trauma models, DNAgel reduced blood loss more effectively than commercial gelatin sponge (GS). In vivo evaluation in a full-thickness skin incision model demonstrated its ability to promote wound healing. Overall, DNAgel shows promise as a rapid hemostatic and wound healing agent.This study introduces a DNA hydrogel (DNAgel) inspired by neutrophil extracellular traps (NETs) to address severe traumatic bleeding. DNAgel is a natural nucleic acid-based material with rapid water absorption, high swelling, and strong tissue adhesion, effectively stopping bleeding. It fills the defect cavity, exerts pressure on bleeding vessels, and promotes coagulation through an artificial DNA scaffold for erythrocyte adhesion and platelet activation. In male rat trauma models, DNAgel reduced blood loss more effectively than commercial gelatin sponge (GS). In vivo evaluation in a full-thickness skin incision model demonstrated its ability to promote wound healing. Overall, DNAgel shows promise as a rapid hemostatic and wound healing agent.