A DNA hydrogel (DNAgel) inspired by neutrophil extracellular traps (NETs) is developed as a wound hemostatic adjuvant. This DNAgel, composed of natural nucleic acids, exhibits rapid water absorption, high swelling, and strong tissue adhesion, enabling it to physically stop bleeding. Its excellent swelling behavior and robust mechanical properties allow it to fill defect cavities and exert pressure on bleeding vessels, achieving compression hemostasis for deep tissue bleeding. The DNAgel also acts as an artificial DNA scaffold for erythrocytes to adhere and aggregate, activating platelets and promoting a bionic coagulation cascade. In rat trauma models, DNAgel reduces blood loss compared to commercial gelatin sponge (GS). In vivo evaluation in a full-thickness skin incision model shows DNAgel promotes wound healing. DNAgel has a high hemostatic capacity and is a promising candidate for rapid hemostasis and wound healing. DNAgel is composed of salmon sperm DNA crosslinked with PEGDA, forming a 3D network with high water absorption, tissue adhesion, and absorption of plasma and cells. It exhibits strong wet tissue adhesion, enabling it to seal wounds and slow bleeding. In vitro tests show DNAgel promotes platelet activation and aggregation, enhancing blood coagulation. In vivo tests demonstrate DNAgel effectively stops bleeding in various models, including rat-tail amputation, femoral artery injury, and liver puncture. DNAgel reduces blood loss significantly compared to GS and control groups. It also promotes wound healing, with faster contraction and healing rates, and shows better tissue regeneration. Histological analysis reveals DNAgel promotes skin regeneration, with intact hair follicles and sebaceous glands. DNAgel has low hemolysis and good blood cell aggregation properties, indicating excellent biocompatibility. It is a promising candidate for clinical use in deep bleeding control and emergency wound care.A DNA hydrogel (DNAgel) inspired by neutrophil extracellular traps (NETs) is developed as a wound hemostatic adjuvant. This DNAgel, composed of natural nucleic acids, exhibits rapid water absorption, high swelling, and strong tissue adhesion, enabling it to physically stop bleeding. Its excellent swelling behavior and robust mechanical properties allow it to fill defect cavities and exert pressure on bleeding vessels, achieving compression hemostasis for deep tissue bleeding. The DNAgel also acts as an artificial DNA scaffold for erythrocytes to adhere and aggregate, activating platelets and promoting a bionic coagulation cascade. In rat trauma models, DNAgel reduces blood loss compared to commercial gelatin sponge (GS). In vivo evaluation in a full-thickness skin incision model shows DNAgel promotes wound healing. DNAgel has a high hemostatic capacity and is a promising candidate for rapid hemostasis and wound healing. DNAgel is composed of salmon sperm DNA crosslinked with PEGDA, forming a 3D network with high water absorption, tissue adhesion, and absorption of plasma and cells. It exhibits strong wet tissue adhesion, enabling it to seal wounds and slow bleeding. In vitro tests show DNAgel promotes platelet activation and aggregation, enhancing blood coagulation. In vivo tests demonstrate DNAgel effectively stops bleeding in various models, including rat-tail amputation, femoral artery injury, and liver puncture. DNAgel reduces blood loss significantly compared to GS and control groups. It also promotes wound healing, with faster contraction and healing rates, and shows better tissue regeneration. Histological analysis reveals DNAgel promotes skin regeneration, with intact hair follicles and sebaceous glands. DNAgel has low hemolysis and good blood cell aggregation properties, indicating excellent biocompatibility. It is a promising candidate for clinical use in deep bleeding control and emergency wound care.