This study investigates the relationship between ankle motion patterns (ankle initial contact angle [AICA] and ankle range of motion [AROM]) and lower limb injury risk during single-leg landing (SL), aiming to optimize landing strategies to reduce injury risk. Sixty healthy male subjects were recruited to collect biomechanics data during SL. The study found that AICA exhibits a negative correlation with peak vertical ground reaction force (PVGRF) and peak ACL force (PAF), and a positive correlation with total energy dissipation (TED) and peak ankle inversion angle (PAIA). AROM shows a positive correlation with TED and PAIA. The results suggest that appropriate increases in AICA (30° to 40°) and AROM (50° to 70°) may reduce lower limb injury risk, particularly ACL injuries, but may increase the risk of lateral ankle sprain (LAS). The study proposes that individuals can consciously adjust their ankle movement patterns during SL to balance the LAS and ACL injury risks, thereby reducing overall injury risk. The findings provide novel insights into optimizing landing strategies and offer theoretical support for decreasing lower limb injury risk during SL.This study investigates the relationship between ankle motion patterns (ankle initial contact angle [AICA] and ankle range of motion [AROM]) and lower limb injury risk during single-leg landing (SL), aiming to optimize landing strategies to reduce injury risk. Sixty healthy male subjects were recruited to collect biomechanics data during SL. The study found that AICA exhibits a negative correlation with peak vertical ground reaction force (PVGRF) and peak ACL force (PAF), and a positive correlation with total energy dissipation (TED) and peak ankle inversion angle (PAIA). AROM shows a positive correlation with TED and PAIA. The results suggest that appropriate increases in AICA (30° to 40°) and AROM (50° to 70°) may reduce lower limb injury risk, particularly ACL injuries, but may increase the risk of lateral ankle sprain (LAS). The study proposes that individuals can consciously adjust their ankle movement patterns during SL to balance the LAS and ACL injury risks, thereby reducing overall injury risk. The findings provide novel insights into optimizing landing strategies and offer theoretical support for decreasing lower limb injury risk during SL.