This study investigates the relationship between ankle motion patterns (ankle initial contact angle [AICA] and ankle range of motion [AROM]) and lower limb injury risk during single-leg landing (SL). The research proposes an optimized landing strategy to reduce injury risk. A knee musculoskeletal model was developed to simulate ACL injury mechanics, incorporating a nonlinear short-term viscoelastic mechanism. Sixty healthy male subjects were tested to collect biomechanics data during SL. Correlation analysis revealed that AICA was negatively correlated with peak vertical ground reaction force (PVGRF) and peak ACL force (PAF), and positively correlated with total energy dissipation (TED) and peak ankle inversion angle (PAIA). AROM was positively correlated with TED and PAIA. The results suggest that increasing AICA (30°–40°) and AROM (50°–70°) may reduce lower limb injury risk. However, this could increase lateral ankle sprain (LAS) risk. The study found an "optimal" ankle motion pattern that balances LAS and ACL injury risk. The findings indicate that appropriate increases in AICA and AROM can reduce injury risk, but further research is needed to confirm these results in female subjects and to consider more detailed indicators of ankle injury risk. The study has potential to provide new insights into optimized landing strategies for reducing lower limb injury risk.This study investigates the relationship between ankle motion patterns (ankle initial contact angle [AICA] and ankle range of motion [AROM]) and lower limb injury risk during single-leg landing (SL). The research proposes an optimized landing strategy to reduce injury risk. A knee musculoskeletal model was developed to simulate ACL injury mechanics, incorporating a nonlinear short-term viscoelastic mechanism. Sixty healthy male subjects were tested to collect biomechanics data during SL. Correlation analysis revealed that AICA was negatively correlated with peak vertical ground reaction force (PVGRF) and peak ACL force (PAF), and positively correlated with total energy dissipation (TED) and peak ankle inversion angle (PAIA). AROM was positively correlated with TED and PAIA. The results suggest that increasing AICA (30°–40°) and AROM (50°–70°) may reduce lower limb injury risk. However, this could increase lateral ankle sprain (LAS) risk. The study found an "optimal" ankle motion pattern that balances LAS and ACL injury risk. The findings indicate that appropriate increases in AICA and AROM can reduce injury risk, but further research is needed to confirm these results in female subjects and to consider more detailed indicators of ankle injury risk. The study has potential to provide new insights into optimized landing strategies for reducing lower limb injury risk.