This study compares two methods for detecting slime production by coagulase-negative staphylococci: the Christensen method and a new Congo red agar method. The Christensen method, which uses tryptic soy broth (TSB), is commonly used but can be less reliable for detecting weak slime production and is subject to interbatch variation. The Congo red agar method, which uses brain heart infusion broth supplemented with 5% sucrose and Congo red stain, was found to be rapid, sensitive, and reproducible. It remains viable on the medium and is less affected by media batch variations. In a comparison of 124 isolates, the Congo red method showed complete agreement with the Christensen method in 107 out of 124 tests, with only one strain being clearly negative by the Christensen method but positive on Congo red agar. The study highlights the advantages of the Congo red method for detecting slime production in coagulase-negative staphylococci.This study compares two methods for detecting slime production by coagulase-negative staphylococci: the Christensen method and a new Congo red agar method. The Christensen method, which uses tryptic soy broth (TSB), is commonly used but can be less reliable for detecting weak slime production and is subject to interbatch variation. The Congo red agar method, which uses brain heart infusion broth supplemented with 5% sucrose and Congo red stain, was found to be rapid, sensitive, and reproducible. It remains viable on the medium and is less affected by media batch variations. In a comparison of 124 isolates, the Congo red method showed complete agreement with the Christensen method in 107 out of 124 tests, with only one strain being clearly negative by the Christensen method but positive on Congo red agar. The study highlights the advantages of the Congo red method for detecting slime production in coagulase-negative staphylococci.