A new method for detecting slime production by coagulase-negative staphylococci (CNS) was compared with the Christensen method on 124 CNS isolates from various clinical sources. The new method uses a specially prepared solid medium containing brain heart infusion broth supplemented with 5% sucrose and Congo red stain. The results showed complete agreement between the two methods in 107 out of 124 tests, with only one strain clearly negative by the Christensen method but positive on Congo red agar. The Congo red method is rapid, sensitive, and reproducible, with the advantage that colonies remain viable on the medium. It is also not subject to interbatch variation of media, which can affect the reproducibility of the Christensen method. The production of slime by CNS is routinely detected using the Christensen method, but it is not always successful for detecting weak slime production. The new method, using Congo red agar, showed better results. The Congo red agar method involves a medium containing brain heart infusion broth, sucrose, agar, and Congo red stain. Positive results are indicated by black colonies with a dry crystalline consistency, while non-slime producers usually remain pink. The new method is more reliable and efficient for detecting slime production. The study highlights the importance of slime production in the pathogenesis of CNS, particularly in patients receiving invasive procedures. The new method provides a more accurate and reliable way to detect slime production, which is important for understanding the virulence and pathogenicity of CNS.A new method for detecting slime production by coagulase-negative staphylococci (CNS) was compared with the Christensen method on 124 CNS isolates from various clinical sources. The new method uses a specially prepared solid medium containing brain heart infusion broth supplemented with 5% sucrose and Congo red stain. The results showed complete agreement between the two methods in 107 out of 124 tests, with only one strain clearly negative by the Christensen method but positive on Congo red agar. The Congo red method is rapid, sensitive, and reproducible, with the advantage that colonies remain viable on the medium. It is also not subject to interbatch variation of media, which can affect the reproducibility of the Christensen method. The production of slime by CNS is routinely detected using the Christensen method, but it is not always successful for detecting weak slime production. The new method, using Congo red agar, showed better results. The Congo red agar method involves a medium containing brain heart infusion broth, sucrose, agar, and Congo red stain. Positive results are indicated by black colonies with a dry crystalline consistency, while non-slime producers usually remain pink. The new method is more reliable and efficient for detecting slime production. The study highlights the importance of slime production in the pathogenesis of CNS, particularly in patients receiving invasive procedures. The new method provides a more accurate and reliable way to detect slime production, which is important for understanding the virulence and pathogenicity of CNS.