The Supporting Information section of the article provides detailed methods and supplementary data for the study of aging-induced transcriptional changes in astrocytes. The methods include TRAP RNA isolation, RNAseq library construction and sequencing, RNAseq analysis, and microfluidics-based qPCR. The RNA was isolated from hippocampi, striati, and cortex of *Aldh11b-eGFP-L10a* mice, and the RNAseq data were used to identify differentially expressed genes between 10-week-old and 2-year-old astrocytes. The analysis involved aligning reads with Bowtie2, identifying splice junctions with TopHat, and estimating expression levels with Cufflinks. Microfluidics-based qPCR was used to validate the RNAseq results, and in situ hybridization was performed to confirm gene expression. The supplementary figures and datasets provide visual and numerical evidence of the transcriptional changes and gene expression patterns in aged astrocytes, including heatmaps, dendrograms, and quantification of gene expression in different brain regions.The Supporting Information section of the article provides detailed methods and supplementary data for the study of aging-induced transcriptional changes in astrocytes. The methods include TRAP RNA isolation, RNAseq library construction and sequencing, RNAseq analysis, and microfluidics-based qPCR. The RNA was isolated from hippocampi, striati, and cortex of *Aldh11b-eGFP-L10a* mice, and the RNAseq data were used to identify differentially expressed genes between 10-week-old and 2-year-old astrocytes. The analysis involved aligning reads with Bowtie2, identifying splice junctions with TopHat, and estimating expression levels with Cufflinks. Microfluidics-based qPCR was used to validate the RNAseq results, and in situ hybridization was performed to confirm gene expression. The supplementary figures and datasets provide visual and numerical evidence of the transcriptional changes and gene expression patterns in aged astrocytes, including heatmaps, dendrograms, and quantification of gene expression in different brain regions.