This review discusses the challenges of treating bacterial biofilms associated with urinary tract infections (UTIs), which are a major cause of healthcare-associated infections. UTIs are common, affecting millions globally, and are often complicated by antibiotic resistance. Biofilms, formed by bacteria on medical devices like urinary catheters, contribute to persistent infections and resistance. The review highlights the importance of innovative treatment approaches due to the limitations of traditional antibiotics. It examines the role of biofilms in UTI pathogenesis, their resistance mechanisms, and the factors contributing to antibiotic resistance. The review also explores alternative therapies, including antimicrobial peptides, nanoparticles, quorum sensing inhibitors, and bacteriophage therapy, which show promise in disrupting biofilms. The study emphasizes the need for new strategies to combat biofilm-associated UTIs, as current treatments are often ineffective against biofilms. The review concludes that understanding biofilm biology and developing targeted therapies are crucial for improving outcomes in UTI management.This review discusses the challenges of treating bacterial biofilms associated with urinary tract infections (UTIs), which are a major cause of healthcare-associated infections. UTIs are common, affecting millions globally, and are often complicated by antibiotic resistance. Biofilms, formed by bacteria on medical devices like urinary catheters, contribute to persistent infections and resistance. The review highlights the importance of innovative treatment approaches due to the limitations of traditional antibiotics. It examines the role of biofilms in UTI pathogenesis, their resistance mechanisms, and the factors contributing to antibiotic resistance. The review also explores alternative therapies, including antimicrobial peptides, nanoparticles, quorum sensing inhibitors, and bacteriophage therapy, which show promise in disrupting biofilms. The study emphasizes the need for new strategies to combat biofilm-associated UTIs, as current treatments are often ineffective against biofilms. The review concludes that understanding biofilm biology and developing targeted therapies are crucial for improving outcomes in UTI management.