This study investigated the association between oral microbiota dysbiosis and oral cavity cancer. Researchers analyzed the oral microbiome of patients with oral cavity cancer and healthy controls using next-generation sequencing of the 16S rRNA gene. The results showed that patients with oral cavity cancer had poorer oral health compared to healthy controls, with significant changes in the composition of their oral microbiome. Specific bacterial genera and species were found to be more abundant in patients with oral cavity cancer, including Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria, while others such as Aggregatibacter, Haemophilus, and Neisseria were decreased. Additionally, major periodontopathogens like Porphyromonas gingivalis and Fusobacterium nucleatum were significantly increased in patients with oral cavity cancer. The study also found that oral health habits, such as tooth brushing and dental care, significantly influenced the oral microbiome. Patients with poor oral hygiene had a higher prevalence of periodontal disease and a less diverse oral microbiome. Smoking and alcohol consumption were also associated with changes in the oral microbiome, with certain bacterial genera decreasing in smokers and alcohol consumers. The study suggests that oral microbiota dysbiosis may be linked to the development and progression of oral cavity cancer. The findings highlight the importance of maintaining good oral hygiene and managing periodontal disease to reduce the risk of oral cavity cancer. The study also emphasizes the need for further research to understand the complex interactions between oral bacteria and the development of oral cancer. The results provide a foundation for future studies exploring the role of oral microbiota in oral cancer diagnosis and treatment.This study investigated the association between oral microbiota dysbiosis and oral cavity cancer. Researchers analyzed the oral microbiome of patients with oral cavity cancer and healthy controls using next-generation sequencing of the 16S rRNA gene. The results showed that patients with oral cavity cancer had poorer oral health compared to healthy controls, with significant changes in the composition of their oral microbiome. Specific bacterial genera and species were found to be more abundant in patients with oral cavity cancer, including Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria, while others such as Aggregatibacter, Haemophilus, and Neisseria were decreased. Additionally, major periodontopathogens like Porphyromonas gingivalis and Fusobacterium nucleatum were significantly increased in patients with oral cavity cancer. The study also found that oral health habits, such as tooth brushing and dental care, significantly influenced the oral microbiome. Patients with poor oral hygiene had a higher prevalence of periodontal disease and a less diverse oral microbiome. Smoking and alcohol consumption were also associated with changes in the oral microbiome, with certain bacterial genera decreasing in smokers and alcohol consumers. The study suggests that oral microbiota dysbiosis may be linked to the development and progression of oral cavity cancer. The findings highlight the importance of maintaining good oral hygiene and managing periodontal disease to reduce the risk of oral cavity cancer. The study also emphasizes the need for further research to understand the complex interactions between oral bacteria and the development of oral cancer. The results provide a foundation for future studies exploring the role of oral microbiota in oral cancer diagnosis and treatment.