The study investigates the origin and development of dendritic cells (DCs) in the intestinal lamina propria (LP), which play a crucial role in mucosal immunity. The research reveals that CX3CR1+CD103+ LP DCs arise exclusively from monocytes under the control of M-CSFR and Flt3 ligands, while CD103+CX3CR1− LP DCs differentiate from common DC progenitors (CDP) and pre-DCs under the influence of Flt3 ligand and GM-CSF. CD103+CX3CR1− DCs, which do not express CCR7, are the first to transport pathogenic *Salmonella* from the intestinal tract to the mesenteric lymph nodes (MLN). The study also highlights the functional differences between the two DC subsets, with CD103+CD11b+ lamina propria DCs being migratory and involved in antigen trafficking and immuno-surveillance, while monocyte-derived CD103+CD11b+ lamina propria DCs have a lower migratory capacity. These findings underscore the diverse origins and functions of the LP DC network, emphasizing the importance of mucosal DCs in gut immune homeostasis.The study investigates the origin and development of dendritic cells (DCs) in the intestinal lamina propria (LP), which play a crucial role in mucosal immunity. The research reveals that CX3CR1+CD103+ LP DCs arise exclusively from monocytes under the control of M-CSFR and Flt3 ligands, while CD103+CX3CR1− LP DCs differentiate from common DC progenitors (CDP) and pre-DCs under the influence of Flt3 ligand and GM-CSF. CD103+CX3CR1− DCs, which do not express CCR7, are the first to transport pathogenic *Salmonella* from the intestinal tract to the mesenteric lymph nodes (MLN). The study also highlights the functional differences between the two DC subsets, with CD103+CD11b+ lamina propria DCs being migratory and involved in antigen trafficking and immuno-surveillance, while monocyte-derived CD103+CD11b+ lamina propria DCs have a lower migratory capacity. These findings underscore the diverse origins and functions of the LP DC network, emphasizing the importance of mucosal DCs in gut immune homeostasis.