PHYML Online is a web interface to the PHYML software, which implements a fast and accurate heuristic for estimating maximum likelihood phylogenies from DNA and protein sequences. This tool offers various options such as nonparametric bootstrap and estimation of evolutionary parameters, enabling comprehensive phylogenetic analyses on large datasets within reasonable computing time. The server, available at http://atgc.lirmm.fr/phyml, supports a wide range of substitution models and allows users to upload multiple datasets and trees for analysis. The algorithm, based on the 'nearest neighbor interchange' operation, efficiently optimizes tree topology and branch lengths, making it suitable for large datasets. Results are provided via email, including a summary of user options, maximum likelihood estimates, and the phylogeny in NEWICK format. PHYML Online is freely available for academic use, with the software also downloadable for local installation.PHYML Online is a web interface to the PHYML software, which implements a fast and accurate heuristic for estimating maximum likelihood phylogenies from DNA and protein sequences. This tool offers various options such as nonparametric bootstrap and estimation of evolutionary parameters, enabling comprehensive phylogenetic analyses on large datasets within reasonable computing time. The server, available at http://atgc.lirmm.fr/phyml, supports a wide range of substitution models and allows users to upload multiple datasets and trees for analysis. The algorithm, based on the 'nearest neighbor interchange' operation, efficiently optimizes tree topology and branch lengths, making it suitable for large datasets. Results are provided via email, including a summary of user options, maximum likelihood estimates, and the phylogeny in NEWICK format. PHYML Online is freely available for academic use, with the software also downloadable for local installation.