PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

(Received for publication, June 1, 1953) | BY R. DULBECCO, M.D., AND MARGUERITE VOGT, M.D.
This paper by Dulbecco and Vogt describes the production of plaques on monolayer cultures of monkey kidney and testis cells infected with poliomyelitis viruses. The authors demonstrate that plaques can be used for titration purposes and that pure lines of viruses can be isolated from single plaques. They also report the successful isolation of high-titer virus stocks. The study highlights the advantages of using monkey kidney cultures due to their uniform susceptibility to the virus. The authors provide detailed methods for preparing tissue cultures, forming monolayers, and infecting cultures with viruses. They show that the number of plaques produced is proportional to the virus concentration and that plaques are specific to the virus type, as evidenced by the suppression of plaque formation by type-specific antiserum. The paper concludes with discussions on the theoretical and practical implications of plaque formation, emphasizing the significance of a single virus particle in initiating infection and the potential for using plaques as a sensitive and reproducible method for virus titration and purification.This paper by Dulbecco and Vogt describes the production of plaques on monolayer cultures of monkey kidney and testis cells infected with poliomyelitis viruses. The authors demonstrate that plaques can be used for titration purposes and that pure lines of viruses can be isolated from single plaques. They also report the successful isolation of high-titer virus stocks. The study highlights the advantages of using monkey kidney cultures due to their uniform susceptibility to the virus. The authors provide detailed methods for preparing tissue cultures, forming monolayers, and infecting cultures with viruses. They show that the number of plaques produced is proportional to the virus concentration and that plaques are specific to the virus type, as evidenced by the suppression of plaque formation by type-specific antiserum. The paper concludes with discussions on the theoretical and practical implications of plaque formation, emphasizing the significance of a single virus particle in initiating infection and the potential for using plaques as a sensitive and reproducible method for virus titration and purification.
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