This research presents an innovative intestinal peristalsis-actuated microneedle robot designed for the oral delivery of biologic drugs. Inspired by the porcupinefish's ability to inflate and deploy spines, the robot is designed to absorb intestinal fluids, expand, and inject drug-loaded microneedles into the intestinal wall. The microneedles, equipped with barbs, can detach from the robot during peristaltic relaxation and remain in the mucosa for sustained drug release. Extensive in vivo experiments using 14 minipigs confirmed the effectiveness of this approach, demonstrating comparable insulin delivery efficacy to subcutaneous injection. The robot's design leverages both mass transport and inherent intestinal peristalsis, eliminating the need for prestored or external energy input. The materials used are biocompatible and biodegradable, making the device suitable for clinical translation. The study highlights the potential of this peristalsis-actuated microneedle robot as a versatile platform for oral drug delivery, offering improved patient compliance and reduced side effects compared to traditional injection methods.This research presents an innovative intestinal peristalsis-actuated microneedle robot designed for the oral delivery of biologic drugs. Inspired by the porcupinefish's ability to inflate and deploy spines, the robot is designed to absorb intestinal fluids, expand, and inject drug-loaded microneedles into the intestinal wall. The microneedles, equipped with barbs, can detach from the robot during peristaltic relaxation and remain in the mucosa for sustained drug release. Extensive in vivo experiments using 14 minipigs confirmed the effectiveness of this approach, demonstrating comparable insulin delivery efficacy to subcutaneous injection. The robot's design leverages both mass transport and inherent intestinal peristalsis, eliminating the need for prestored or external energy input. The materials used are biocompatible and biodegradable, making the device suitable for clinical translation. The study highlights the potential of this peristalsis-actuated microneedle robot as a versatile platform for oral drug delivery, offering improved patient compliance and reduced side effects compared to traditional injection methods.