Pericytes (PCs) are cells surrounding small blood vessels, particularly capillaries and microvessels, and play crucial roles in maintaining vascular integrity, angiogenesis, and blood flow regulation. They also serve as a reservoir for multipotent stem/progenitor cells in various adipose tissues, including white, brown, beige, and bone marrow adipose tissues. The heterogeneity of PCs makes their identification and characterization challenging, but understanding their complex nature is essential for therapeutic applications in metabolic syndromes and bone-related diseases. This review summarizes the commonly used markers for lineage-tracing studies of PCs, focusing on their contributions to adipogenesis and functions in different adipose depots under diverse metabolic conditions. PCs have been implicated in angiogenesis, tissue regeneration, and adipocyte differentiation, with specific markers such as PDGFRβ, NG2, TBX18, α-SMA, NESTIN, and CD146 being used for profiling and lineage tracing. The review also discusses the role of PCs in the bone marrow niche, where they influence stromal cell proliferation, hematopoietic stem cell maintenance, and osteoblast and adipocyte differentiation. Overall, PCs are multifaceted cells that orchestrate a series of functions crucial for adipose tissue homeostasis, and their manipulation holds potential for therapeutic interventions in conditions related to adipose tissue dysfunction.Pericytes (PCs) are cells surrounding small blood vessels, particularly capillaries and microvessels, and play crucial roles in maintaining vascular integrity, angiogenesis, and blood flow regulation. They also serve as a reservoir for multipotent stem/progenitor cells in various adipose tissues, including white, brown, beige, and bone marrow adipose tissues. The heterogeneity of PCs makes their identification and characterization challenging, but understanding their complex nature is essential for therapeutic applications in metabolic syndromes and bone-related diseases. This review summarizes the commonly used markers for lineage-tracing studies of PCs, focusing on their contributions to adipogenesis and functions in different adipose depots under diverse metabolic conditions. PCs have been implicated in angiogenesis, tissue regeneration, and adipocyte differentiation, with specific markers such as PDGFRβ, NG2, TBX18, α-SMA, NESTIN, and CD146 being used for profiling and lineage tracing. The review also discusses the role of PCs in the bone marrow niche, where they influence stromal cell proliferation, hematopoietic stem cell maintenance, and osteoblast and adipocyte differentiation. Overall, PCs are multifaceted cells that orchestrate a series of functions crucial for adipose tissue homeostasis, and their manipulation holds potential for therapeutic interventions in conditions related to adipose tissue dysfunction.