A bioink composed of silk fibroin (SF) and iota-carrageenan (CG) was developed to enhance printability in extrusion-based bioprinting. The SF/CG ink exhibited suitable viscosity and shear-thinning properties, along with the rapid sol-gel transition of CG. Photo-crosslinking of SF significantly improved printability and structural integrity of 3D constructs. The printed constructs had a Young's modulus of approximately 250 kPa, suitable for keratinocyte and myoblast cell culture. The high cell adhesiveness and viability (maximum >98%) of the loaded cells demonstrated the potential of this 3D culture scaffold for skin and muscle tissues. The SF/CG bioink was optimized for extrusion-based bioprinting, showing superior printability with maintained rheological properties and creating a microenvironment conducive to desired cellular behavior. The constructs closely resembled native tissues, ensuring high shape fidelity. The bioink was evaluated for its applicability in tissue regeneration applications. The SF/CG bioink was found to have excellent mechanical properties, with the SF91CG9 construct showing the highest tensile strength. The bioink also exhibited good biocompatibility, with high cell viability and minimal cytotoxicity. The SF/CG bioink was found to be suitable for extrusion-based bioprinting, enabling the fabrication of complex 3D structures with high resolution and reproducibility. The study demonstrated the potential of the SF/CG bioink for tissue engineering applications.A bioink composed of silk fibroin (SF) and iota-carrageenan (CG) was developed to enhance printability in extrusion-based bioprinting. The SF/CG ink exhibited suitable viscosity and shear-thinning properties, along with the rapid sol-gel transition of CG. Photo-crosslinking of SF significantly improved printability and structural integrity of 3D constructs. The printed constructs had a Young's modulus of approximately 250 kPa, suitable for keratinocyte and myoblast cell culture. The high cell adhesiveness and viability (maximum >98%) of the loaded cells demonstrated the potential of this 3D culture scaffold for skin and muscle tissues. The SF/CG bioink was optimized for extrusion-based bioprinting, showing superior printability with maintained rheological properties and creating a microenvironment conducive to desired cellular behavior. The constructs closely resembled native tissues, ensuring high shape fidelity. The bioink was evaluated for its applicability in tissue regeneration applications. The SF/CG bioink was found to have excellent mechanical properties, with the SF91CG9 construct showing the highest tensile strength. The bioink also exhibited good biocompatibility, with high cell viability and minimal cytotoxicity. The SF/CG bioink was found to be suitable for extrusion-based bioprinting, enabling the fabrication of complex 3D structures with high resolution and reproducibility. The study demonstrated the potential of the SF/CG bioink for tissue engineering applications.