Phylogeny.fr is a web-based platform that provides robust phylogenetic analysis tools for both non-specialists and experts. It offers three main modes: 'One Click', 'Advanced', and 'A la Carte'. The 'One Click' mode is designed for users with no experience in phylogeny, providing a ready-to-use pipeline with MUSCLE for multiple alignment, PhyML for tree building, and TreeDyn for tree rendering. The 'Advanced' mode allows customization of parameters, while the 'A la Carte' mode offers flexibility for users to build their own pipelines. The platform also includes a Blast module for sequence searching and a guide tree to help select neighbor sequences. Phylogeny.fr supports both DNA and protein sequences and offers various tools for phylogenetic analysis, including maximum likelihood, parsimony, and distance-based methods. The platform is user-friendly, with a tabulated menu and examples to guide users. It also provides visualization tools for tree images and allows users to manipulate and edit results. Phylogeny.fr is available at http://www.phylogeny.fr/ and is designed to be a comprehensive and flexible solution for phylogenetic analysis. The platform is supported by a dedicated server and a 25-node Linux cluster for Blast processing. It has a modular architecture that allows for the addition of new features and programs as the field evolves. Phylogeny.fr is not committed to a particular set of programs and will replace outdated methods with newer ones if they are considered better by the scientific community. The platform is currently used by about 800 users, processing around 2500 submissions per month. It is expected to improve its capabilities in the future, including the ability to select the best substitution model and provide password-protected accounts for users to store and load results.Phylogeny.fr is a web-based platform that provides robust phylogenetic analysis tools for both non-specialists and experts. It offers three main modes: 'One Click', 'Advanced', and 'A la Carte'. The 'One Click' mode is designed for users with no experience in phylogeny, providing a ready-to-use pipeline with MUSCLE for multiple alignment, PhyML for tree building, and TreeDyn for tree rendering. The 'Advanced' mode allows customization of parameters, while the 'A la Carte' mode offers flexibility for users to build their own pipelines. The platform also includes a Blast module for sequence searching and a guide tree to help select neighbor sequences. Phylogeny.fr supports both DNA and protein sequences and offers various tools for phylogenetic analysis, including maximum likelihood, parsimony, and distance-based methods. The platform is user-friendly, with a tabulated menu and examples to guide users. It also provides visualization tools for tree images and allows users to manipulate and edit results. Phylogeny.fr is available at http://www.phylogeny.fr/ and is designed to be a comprehensive and flexible solution for phylogenetic analysis. The platform is supported by a dedicated server and a 25-node Linux cluster for Blast processing. It has a modular architecture that allows for the addition of new features and programs as the field evolves. Phylogeny.fr is not committed to a particular set of programs and will replace outdated methods with newer ones if they are considered better by the scientific community. The platform is currently used by about 800 users, processing around 2500 submissions per month. It is expected to improve its capabilities in the future, including the ability to select the best substitution model and provide password-protected accounts for users to store and load results.