Phytozome is a comprehensive comparative platform for green plant genomics, providing a centralized hub for plant genome and gene family data and analysis. It offers a view of the evolutionary history of plant genes at various levels, including sequence, gene structure, gene family, and genome organization. Phytozome supports access to sequences and functional annotations of 25 complete plant genomes, including all land plants and selected algae sequenced at the Joint Genome Institute, as well as other sequenced species. The platform integrates various open-source components and custom visualization tools, enabling users to perform gene family searches, inspect and evaluate gene families, and assign putative functions to uncharacterized sequences. Phytozome also provides genome-centric views through GBrowse, allowing users to explore genomic contexts and alignments. The content of Phytozome is regularly updated, with new genomes and features added annually, and the platform is designed to facilitate comparative genomics research in plant science.Phytozome is a comprehensive comparative platform for green plant genomics, providing a centralized hub for plant genome and gene family data and analysis. It offers a view of the evolutionary history of plant genes at various levels, including sequence, gene structure, gene family, and genome organization. Phytozome supports access to sequences and functional annotations of 25 complete plant genomes, including all land plants and selected algae sequenced at the Joint Genome Institute, as well as other sequenced species. The platform integrates various open-source components and custom visualization tools, enabling users to perform gene family searches, inspect and evaluate gene families, and assign putative functions to uncharacterized sequences. Phytozome also provides genome-centric views through GBrowse, allowing users to explore genomic contexts and alignments. The content of Phytozome is regularly updated, with new genomes and features added annually, and the platform is designed to facilitate comparative genomics research in plant science.