This study presents a novel biomimetic scaffold combining piezoelectric and topographical properties with hydroxyapatite (HAp) to accelerate bone regeneration. The scaffold is fabricated by incorporating HAp into polyvinylidene fluoride-co-trifluoroethylene (P(VDF-TrFE)) in a freestanding form, leveraging HAp's natural osteogenic potential. The scaffold's unique design enables the simultaneous delivery of electrical, topographical, and paracrine cues that promote bone healing. In vitro and in vivo experiments demonstrate that the scaffold significantly enhances bone regeneration, with improved cell proliferation, osteogenic differentiation, and mineralization. The scaffold's piezoelectric properties, enhanced by HAp, generate electrical signals in response to mechanical stress, while its topographical features mimic the extracellular matrix, promoting cell adhesion and function. Additionally, the scaffold's paracrine effects are mediated through the secretion of growth factors that support bone regeneration. The study highlights the synergistic effects of piezoelectric and topographical cues in enhancing bone regeneration and proposes a new strategy for designing biomimetic scaffolds for bone tissue engineering. The findings suggest that HAp/P(VDF-TrFE) scaffolds have broad applications in regenerative medicine due to their excellent mechanical, electrical, and biomimetic properties.This study presents a novel biomimetic scaffold combining piezoelectric and topographical properties with hydroxyapatite (HAp) to accelerate bone regeneration. The scaffold is fabricated by incorporating HAp into polyvinylidene fluoride-co-trifluoroethylene (P(VDF-TrFE)) in a freestanding form, leveraging HAp's natural osteogenic potential. The scaffold's unique design enables the simultaneous delivery of electrical, topographical, and paracrine cues that promote bone healing. In vitro and in vivo experiments demonstrate that the scaffold significantly enhances bone regeneration, with improved cell proliferation, osteogenic differentiation, and mineralization. The scaffold's piezoelectric properties, enhanced by HAp, generate electrical signals in response to mechanical stress, while its topographical features mimic the extracellular matrix, promoting cell adhesion and function. Additionally, the scaffold's paracrine effects are mediated through the secretion of growth factors that support bone regeneration. The study highlights the synergistic effects of piezoelectric and topographical cues in enhancing bone regeneration and proposes a new strategy for designing biomimetic scaffolds for bone tissue engineering. The findings suggest that HAp/P(VDF-TrFE) scaffolds have broad applications in regenerative medicine due to their excellent mechanical, electrical, and biomimetic properties.