Plant-derived exosome-like nanoparticles (ELNs) are emerging as promising nanosystems for tissue engineering. These nanoparticles, derived from plants, exhibit unique properties such as biocompatibility, low immunogenicity, and the ability to deliver bioactive molecules, making them valuable for tissue regeneration. They can modulate immune responses, promote cell proliferation and differentiation, and support angiogenesis, which are crucial for tissue repair. ELNs can be engineered to target specific cells, enhancing their therapeutic potential. Their ability to carry and release various bioactive cargoes, including nucleic acids, proteins, and lipids, allows them to influence cellular processes and create a favorable environment for tissue regeneration. ELNs have shown potential in various tissue engineering applications, including bone, cartilage, and wound healing. They can enhance osteogenic differentiation, promote cartilage regeneration, and accelerate wound healing by modulating inflammatory responses and supporting cell migration and proliferation. Additionally, their surface can be modified to improve biocompatibility and reduce immune reactions, making them suitable for clinical applications. Despite their promising potential, challenges remain in the commercialization of plant-derived ELNs, including standardization of production processes, ensuring consistent quality, and addressing toxicological concerns. However, ongoing research and development are paving the way for their successful application in regenerative medicine. The future of plant-derived ELNs in tissue engineering is bright, with continued efforts to overcome existing challenges and realize their full therapeutic potential.Plant-derived exosome-like nanoparticles (ELNs) are emerging as promising nanosystems for tissue engineering. These nanoparticles, derived from plants, exhibit unique properties such as biocompatibility, low immunogenicity, and the ability to deliver bioactive molecules, making them valuable for tissue regeneration. They can modulate immune responses, promote cell proliferation and differentiation, and support angiogenesis, which are crucial for tissue repair. ELNs can be engineered to target specific cells, enhancing their therapeutic potential. Their ability to carry and release various bioactive cargoes, including nucleic acids, proteins, and lipids, allows them to influence cellular processes and create a favorable environment for tissue regeneration. ELNs have shown potential in various tissue engineering applications, including bone, cartilage, and wound healing. They can enhance osteogenic differentiation, promote cartilage regeneration, and accelerate wound healing by modulating inflammatory responses and supporting cell migration and proliferation. Additionally, their surface can be modified to improve biocompatibility and reduce immune reactions, making them suitable for clinical applications. Despite their promising potential, challenges remain in the commercialization of plant-derived ELNs, including standardization of production processes, ensuring consistent quality, and addressing toxicological concerns. However, ongoing research and development are paving the way for their successful application in regenerative medicine. The future of plant-derived ELNs in tissue engineering is bright, with continued efforts to overcome existing challenges and realize their full therapeutic potential.