The article reviews the emerging field of plant-derived exosome-like nanoparticles (ELNs) in tissue engineering, highlighting their potential for enhancing tissue repair and regeneration. ELNs, characterized by their biocompatibility, immunomodulatory properties, and ability to deliver bioactive molecules, show promise in various applications such as bone, cartilage, and wound healing. The review covers the properties, isolation techniques, and biological activities of ELNs, emphasizing their role in promoting cell proliferation, differentiation, and angiogenesis. It also discusses strategies to enhance biocompatibility and minimize immune reactions, and explores the commercialization and industrial perspectives of ELNs. Despite challenges in production and standardization, the authors conclude that ELNs hold significant potential for advancing regenerative medicine and tissue engineering.The article reviews the emerging field of plant-derived exosome-like nanoparticles (ELNs) in tissue engineering, highlighting their potential for enhancing tissue repair and regeneration. ELNs, characterized by their biocompatibility, immunomodulatory properties, and ability to deliver bioactive molecules, show promise in various applications such as bone, cartilage, and wound healing. The review covers the properties, isolation techniques, and biological activities of ELNs, emphasizing their role in promoting cell proliferation, differentiation, and angiogenesis. It also discusses strategies to enhance biocompatibility and minimize immune reactions, and explores the commercialization and industrial perspectives of ELNs. Despite challenges in production and standardization, the authors conclude that ELNs hold significant potential for advancing regenerative medicine and tissue engineering.