This review provides a comprehensive overview of the pharmacological potential of silver nanoparticles (AgNPs) synthesized using plant-mediated methods. AgNPs, due to their unique properties, have shown significant promise in various biomedical applications, including antimicrobial, cytotoxic, wound healing, larvicidal, anti-angiogenesis, antioxidant, and antiplasmodial activities. The synthesis of AgNPs using plant extracts is considered a sustainable and eco-friendly approach, offering advantages such as cost-effectiveness, reduced synthesis time, and minimal environmental impact. The review discusses the mechanisms underlying the pharmacological actions of AgNPs, including their interactions with biological systems, their ability to disrupt cell membranes, generate reactive oxygen species, and induce DNA damage. The review also highlights the importance of characterization techniques such as UV-Vis spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy, transmission electron microscopy, dynamic light scattering, zeta potential analysis, and X-ray photoelectron spectroscopy in understanding the properties and behavior of AgNPs. The review emphasizes the potential of AgNPs in various medical applications, including antimicrobial therapy, cancer treatment, wound healing, and bone healing. The review also discusses the challenges associated with the use of AgNPs, such as their potential toxicity and the need for further research to optimize their therapeutic applications. Overall, the review underscores the importance of AgNPs in the field of nanomedicine and their potential to revolutionize various areas of healthcare and technology.This review provides a comprehensive overview of the pharmacological potential of silver nanoparticles (AgNPs) synthesized using plant-mediated methods. AgNPs, due to their unique properties, have shown significant promise in various biomedical applications, including antimicrobial, cytotoxic, wound healing, larvicidal, anti-angiogenesis, antioxidant, and antiplasmodial activities. The synthesis of AgNPs using plant extracts is considered a sustainable and eco-friendly approach, offering advantages such as cost-effectiveness, reduced synthesis time, and minimal environmental impact. The review discusses the mechanisms underlying the pharmacological actions of AgNPs, including their interactions with biological systems, their ability to disrupt cell membranes, generate reactive oxygen species, and induce DNA damage. The review also highlights the importance of characterization techniques such as UV-Vis spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy, transmission electron microscopy, dynamic light scattering, zeta potential analysis, and X-ray photoelectron spectroscopy in understanding the properties and behavior of AgNPs. The review emphasizes the potential of AgNPs in various medical applications, including antimicrobial therapy, cancer treatment, wound healing, and bone healing. The review also discusses the challenges associated with the use of AgNPs, such as their potential toxicity and the need for further research to optimize their therapeutic applications. Overall, the review underscores the importance of AgNPs in the field of nanomedicine and their potential to revolutionize various areas of healthcare and technology.