This review discusses plant growth regulation in cell and tissue culture in vitro, emphasizing the importance of understanding factors that influence plant tissue culture and regeneration. The authors highlight the role of auxin, biotechnology, culture media, and somatic embryogenesis in plant regeneration. They describe various regenerative pathways, including organogenesis, somatic embryogenesis, haploid induction, and protoplast culture. The review also covers nutrient balance in vitro, detailing the roles of macro and microelements, halogens, and organic components in plant tissue culture. The authors emphasize the importance of optimizing culture conditions, including pH, light, and stress factors, to enhance plant regeneration. The review provides practical recommendations based on extensive research and experience in plant biotechnology. The main aim is to help new plant biotechnologists and improve the impact of the plant tissue culture industry globally.This review discusses plant growth regulation in cell and tissue culture in vitro, emphasizing the importance of understanding factors that influence plant tissue culture and regeneration. The authors highlight the role of auxin, biotechnology, culture media, and somatic embryogenesis in plant regeneration. They describe various regenerative pathways, including organogenesis, somatic embryogenesis, haploid induction, and protoplast culture. The review also covers nutrient balance in vitro, detailing the roles of macro and microelements, halogens, and organic components in plant tissue culture. The authors emphasize the importance of optimizing culture conditions, including pH, light, and stress factors, to enhance plant regeneration. The review provides practical recommendations based on extensive research and experience in plant biotechnology. The main aim is to help new plant biotechnologists and improve the impact of the plant tissue culture industry globally.