Plant growth-promoting rhizobacteria (PGPR) significantly enhance the growth and quality of several crops, including tea, tobacco, and chili peppers. Three PGPR strains—FJS-3 (Burkholderia pyromania), FJS-7 (Pseudomonas rhodesiae), and FJS-16 (Pseudomonas baetica)—were tested in greenhouse and field experiments. In greenhouse trials, PGPR treatments increased the growth of tea seedlings, tobacco, and chili peppers compared to controls. For tea, PGPR application led to a 41.03% increase in new shoots and 12.17% in plant height. For tobacco, FJS-3 showed the best growth promotion, increasing plant height, root weight, and fresh weight by 25.56%, 24.77%, and 21.21%, respectively. Multi-strain PGPR application further enhanced growth, with tobacco plant height, fresh weight, and root weight increasing by 30.15%, 37.36%, and 54.5%, respectively. In field trials in Guizhou, PGPR application with compound fertilizer (T2) significantly increased tea yield and quality. Compared to no fertilizer (T1), T2 increased tea bud weight and germination density by 18.46% and 20.18%, respectively. Tea yield under T2 was 125.06% higher than T1, with improved tea quality, including increased theanine content and reduced caffeine and polyphenol levels. PGPR also increased chlorophyll content, enhancing tea color and appearance. Sensory evaluations confirmed that T2 tea had the best taste and appearance. PGPR application improved tea quality by enhancing theanine content, reducing caffeine and polyphenol levels, and increasing chlorophyll content. These changes improved the umami flavor and reduced bitterness, aligning with matcha quality standards. PGPR also reduced the need for chemical fertilizers, promoting sustainable agriculture. The study demonstrates that PGPR can effectively promote plant growth and improve crop quality, with significant potential for application in agriculture. The results highlight the importance of PGPR in sustainable farming practices, offering a promising alternative to chemical fertilizers.Plant growth-promoting rhizobacteria (PGPR) significantly enhance the growth and quality of several crops, including tea, tobacco, and chili peppers. Three PGPR strains—FJS-3 (Burkholderia pyromania), FJS-7 (Pseudomonas rhodesiae), and FJS-16 (Pseudomonas baetica)—were tested in greenhouse and field experiments. In greenhouse trials, PGPR treatments increased the growth of tea seedlings, tobacco, and chili peppers compared to controls. For tea, PGPR application led to a 41.03% increase in new shoots and 12.17% in plant height. For tobacco, FJS-3 showed the best growth promotion, increasing plant height, root weight, and fresh weight by 25.56%, 24.77%, and 21.21%, respectively. Multi-strain PGPR application further enhanced growth, with tobacco plant height, fresh weight, and root weight increasing by 30.15%, 37.36%, and 54.5%, respectively. In field trials in Guizhou, PGPR application with compound fertilizer (T2) significantly increased tea yield and quality. Compared to no fertilizer (T1), T2 increased tea bud weight and germination density by 18.46% and 20.18%, respectively. Tea yield under T2 was 125.06% higher than T1, with improved tea quality, including increased theanine content and reduced caffeine and polyphenol levels. PGPR also increased chlorophyll content, enhancing tea color and appearance. Sensory evaluations confirmed that T2 tea had the best taste and appearance. PGPR application improved tea quality by enhancing theanine content, reducing caffeine and polyphenol levels, and increasing chlorophyll content. These changes improved the umami flavor and reduced bitterness, aligning with matcha quality standards. PGPR also reduced the need for chemical fertilizers, promoting sustainable agriculture. The study demonstrates that PGPR can effectively promote plant growth and improve crop quality, with significant potential for application in agriculture. The results highlight the importance of PGPR in sustainable farming practices, offering a promising alternative to chemical fertilizers.