This research article introduces a novel dual-channel single-polarization photonic crystal fiber (PCF) sensor for multi-analyte detection. The sensor, fabricated using the stack-and-draw technique, offers high sensitivity and real-time measurement capabilities. It demonstrates maximum wavelength and amplitude sensitivities of 11,000 nm/RIU and 807 RIU-1, respectively, for analyte refractive indices (RI) ranging from 1.33 to 1.41. The sensor's design features two large channels near the core, allowing for high birefringence and single polarization, which enhances its performance. Numerical simulations using COMSOL Multiphysics show that the sensor can detect multiple analytes simultaneously, with minimal channel dependency on analyte RI. The sensor's performance is further optimized by adjusting the gold layer thickness, achieving optimal results at 70 nm. Compared to existing sensors, the proposed DCSP sensor exhibits superior performance, making it suitable for applications in disease detection and industrial chemical analysis.This research article introduces a novel dual-channel single-polarization photonic crystal fiber (PCF) sensor for multi-analyte detection. The sensor, fabricated using the stack-and-draw technique, offers high sensitivity and real-time measurement capabilities. It demonstrates maximum wavelength and amplitude sensitivities of 11,000 nm/RIU and 807 RIU-1, respectively, for analyte refractive indices (RI) ranging from 1.33 to 1.41. The sensor's design features two large channels near the core, allowing for high birefringence and single polarization, which enhances its performance. Numerical simulations using COMSOL Multiphysics show that the sensor can detect multiple analytes simultaneously, with minimal channel dependency on analyte RI. The sensor's performance is further optimized by adjusting the gold layer thickness, achieving optimal results at 70 nm. Compared to existing sensors, the proposed DCSP sensor exhibits superior performance, making it suitable for applications in disease detection and industrial chemical analysis.